2.) Which of these is not a restriction enzyme?
A) Bam HI
B) HIndIII
C) pAMP
pAMP is not the restriction enzyme.
Option C)
* restriction enzyme are produced by those bacteria who has the property of cleaving DNA molecule near specific Recognition sites
2.) Which of these is not a restriction enzyme? A) Bam HI B) HIndIII C) pAMP
Which restriction enzyme is best for cloning in pUC8? Pst I Hind III Bam HI Eco RI endonuclease
True or false: A piece of DNA cut with a restriction enzyme such as Bam H1 is unlikely to be successfully ligated into a plasmid that has been cut with a different enzyme such as EcoR1.
If the target DNA (the 3.266 Kb E. coli genomic Bam HI fragment)
has the same restriction sites on each end, there are two possible
orientations for the target DNA to insert into the plasmid. The
following restriction enzymes would cut the 3.266 kb Bam HI genomic
fragment containing the RecA gene once or twice or not at all. In
the tables below, list the expected DNA fragment sizes for the two
possible orientations. Round the DNA fragment sizes to...
The first three bases of the 6-base recognition cleavage site of the restriction enzyme, HindIII are AAG. What is the complete sequence of this 6 bp site? Provide an explanation for your answer.
Would a Bam HI restriction Digest of your suspect DNA look the same as a Pst I digest of it. Why or Why not.
Assume that you have cut λ DNA with the restriction enzyme HindIII. You separate the fragments on an agarose gel and stain the DNA with ethidium bromide. You notice that the intensity of the stain is less in the bands that have migrated closer to the "+" pole. Give an explanation for this finding.
1.) If we mix two restriction fragments cut with the same restriction enzyme in the presence of DNA ligase, ATP and appropriate buffer, we will obtain... A) protein B) carbohydrate C) recombinant DNA or plasmid D) pAMP
Which of the following restriction enzymes do(es) generate sticky ends? A) Enzyme Recognition BamHI G↓GATCC CCTAG↑G B) Enzyme Recognition EcoRI G↓AATTC CTTAA↑G C) Enzyme Recognition HaeIII GG↓CC CC↑GG D) Enzyme Recognition HindIII A↓AGCTT TTCGA↑A E) all of the above, except c
Which of the following restriction endonucleases produces 3' overhangs? (position where the enzyme cuts is indicated by an arrow) A. EcoRI (5'-G↓AATTC-3') B. HindIII (5'-A↓AGCTT-3') C. EcoRV (5'-GAT↓ATC-3') D. PstI (5'-CTGCA↓G-3')
HindIII 5000, 2500 SmaI 5500, 2000 EcoRI 4000, 3500 HindIII + EcoRI 3500, 2500, 1500 HindIII + SmaI 3000, 2500, 2000 SmaI + EcoRI 4000, 2000, 1500 23. The enzyme PspN4I cleaves the sequence NNGCNN (N= any of the four bases). How many times would you expect PspN4I to digest a 10.752 kb genome? Sequence A, which contains two BstBI sites (TT/CGAA), was digested with BstBI. The resulting fragment was then ligated into the unique TaqI restriction site (T/CGA) within...