HomeworkLib
Question

Which restriction enzyme is best for cloning in pUC8? Pst I Hind III Bam HI Eco...

Which restriction enzyme is best for cloning in pUC8?

Pst I

Hind III

Bam HI

Eco RI endonuclease

science   biology   
0 0
Add a comment Improve this question Transcribed image text
Next > < Previous
Sort answers by oldest

Homework Answers

Answer #1

Option d is correct. Eco RI endonuclease is best for cloning in pUC8. This is because the plasmid can easily reform into a circle witjwit a DNA insert due to its complementarity.

0 0
Add a comment
Know the answer?
Add Answer to:
Which restriction enzyme is best for cloning in pUC8? Pst I Hind III Bam HI Eco...
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for? Ask your own homework help question. Our experts will answer your question WITHIN MINUTES for Free.
Similar Homework Help Questions

  • Would a Bam HI restriction Digest of your suspect DNA look the same as a Pst...

    Would a Bam HI restriction Digest of your suspect DNA look the same as a Pst I digest of it. Why or Why not.

  • 2.) Which of these is not a restriction enzyme? A) Bam HI B) HIndIII C) pAMP

    2.) Which of these is not a restriction enzyme? A) Bam HI B) HIndIII C) pAMP

  • The figure below shows a restriction map of a segment of a DNA molecule. Eco refers...

    The figure below shows a restriction map of a segment of a DNA molecule. Eco refers to locations where the restriction endonuclease EcoRI cuts the DNA, and Pst refers to locations where the restriction enzyme Pst cuts the DNA. Potential restriction sites are numbered 1-6. Distances between restriction sites are shown on the bottom scale in base pairs (bp). The thick line represents the part of the molecule that has homology with a probe. Eco Pst Eco Pst Eco Pst...

  • on the me but not with the larger fragments on o t and more and record...

    on the me but not with the larger fragments on o t and more and record med in the h 3. Determine the distracted for each bund data bere 4. Determine the distance traveled for each and generated in the double digest and record data bere tion Endonuclease Digestion and Gel Electrophoresis of DNA 185 VI. RESTRICTION MAPPING The different DNA fragments generated by different map DNA. For example, specific DNA on 2000 nucleotides in las Rl or Hind and...

  • A DNA molecule is cleaved with Hind III, Pst II, or the two enzymes together. The...

    A DNA molecule is cleaved with Hind III, Pst II, or the two enzymes together. The accompanying diagram shows the resulting electrophoresis gel, with band sizes indicated. Is the DNA molecule in question linear or circular? Linear circular Using the information in question 6. determine where the Pst 1 restriction site is located. Pstl cleave within the 7kb HindM fragment or within the 3kb HindM fragment'; 7kb fragment 3kb fragment neither fragment both fragments

  • 1.When cloning a PCR product into a plasmid using restriction enzymes, the restriction enzyme recognition sequences...

    1.When cloning a PCR product into a plasmid using restriction enzymes, the restriction enzyme recognition sequences in the PCR product most likely came from _______, and the restriction enzyme recognition sequences in the plasmid most likely came from ________. a. A multiple cloning site / the primers b. The primers / a multiple cloning site c. Both came from primers d. Both came from the multiple cloning site e. Naturally present in the gene of interest / the multiple cloning...

  • A linear fragment of DNA is cleaved with the individual restriction enzymes Hindill and Smal, and...

    A linear fragment of DNA is cleaved with the individual restriction enzymes Hindill and Smal, and then with a combination of the two enzymes. The fragments obtained are: Eco RV 2 kb, 3.5 kb, 9.5 kb Bam HI 6 kb, 9 kb Eco RV and Bam H12 kb, 3.5 kb, 4 kb. 5.5 kb Draw a restriction map of the DNA fragment. (5 marks). Model of example restriction map to show you how to give your answer: Eco R1 Hind...

  • L= No restriction B=Bam HI E=EcoRI H=Hind III Band 1 27mm 31mm 29mm 29mm Band 2...

    L= No restriction B=Bam HI E=EcoRI H=Hind III Band 1 27mm 31mm 29mm 29mm Band 2 NA 34mm 41mm 37mm Band 3 NA 41mm 46mm 43mm Band 4 NA 43mm 49mm 52mm Band 5 NA 46mm 57mm 71mm Band 6 NA NA NA 76mm Above is the actual measurements for the distance in mm. Please plug this in with the existing chart located above Gel Electrophoresis lab assignment The following sheets will be used to demonstrate your knowledge of gel...

  • PART 3 -SCREENING POTENTIAL CLONES (2 MARKS). After completing a cloning experiment, we screen potential clones...

    PART 3 -SCREENING POTENTIAL CLONES (2 MARKS). After completing a cloning experiment, we screen potential clones to determine which ones are successful. The experiments outlined in Session 2-4 in the lab manual describe how we do this for the LEAPS plasmid. In this experiment using just the EcoRI site to facilitate cloning we have an additional problem - both ends of the PCR product have an EcoRI site and this means that the amplified sequence can be cloned into the...

  • Use the following depiction of a gel of Hind III fragments to answer the questions: 1...

    Use the following depiction of a gel of Hind III fragments to answer the questions: 1 Kb Ladder Possible Hind III Fragments: 1 Kb Ladder Sizes 10,000 8,000 564 bp 8,888 2.0 kb 2.3 kb 11 4.36 kb 4,000 3,000 2,500 2,000 1,500 6.5 kb 9.4 kb 23 kb 1,000 750 500 250 1. Based on the gel results, list the sizes of the chromosome fragments cloned: 2. Based on the gel results, list the sizes of the chromosome fragments...

ADVERTISEMENT
Free Homework Help App
Download From Google Play
Scan Your Homework
to Get Instant Free Answers
Need Online Homework Help?
Ask a Question
Get Answers For Free
Most questions answered within 3 hours.
ADVERTISEMENT
ADVERTISEMENT