Let-7 is a partially complementary miRNA that regulates the expression of lin-41 in C.elegans (worms). The...
Let-7 is a partially complementary miRNA that regulates the expression of lin-41 in C.elegans (worms). The let-7 sequence is deleted in mutant worms. In the mutant worms (compared to
normal worms), what amounts of the following would you expect to see?
a. Let-7 miRNA
b. Lin-41 mRNA
c. Lin-41 protein
(a. is less, b. is the same amount, and c. is more, but why?)
Homework Answers
When the gene for Let-7 miRNA is deleted in mutant worms, expression of Let-7 miRNA would be comparatively less in the mutant worms. Since Let-7 miRNA is responsible for regulating the expression of lin-41, in mutant worms, we can expect an increase in the expression of lin-41 protein. Because, when there is not enough let-7 miRNA to regulate, the expression of lin-41 would go unregulated. But since let-7 miRNA is not affecting the transcription of lin-41 gene, the concentration of lin-41 miRNA is expected to remain the same.
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Let-7 is a partially complementary miRNA that regulates the
expression of lin-41 in C.elegans (worms). The...
1 2 3 4 5 lane lane (fig60) -- Lin-41 western blot let-7 northern blot The...
1 2 3 4 5 lane lane (fig60) -- Lin-41 western blot let-7 northern blot The images above show a northern blot using a probe to identify the let-7 miRNA (left), and a western blot (right) with a primary antibody to recognize Lin-41, a let-7 target. Assume each lane in both the northern and western blots come from the same sample. Which lane best matches each scenario described listed below? Normally processed let-7 miRNA let-7 miRNA in a Drosha mutant...
7-41 Which of the following pairs of codons might you expect to be read by the...
7-41 Which of the following pairs of codons might you expect to be read by the same tRNA as a result of wobble? (a) (b) CUU and UUU GAU and GAA CAC and CAU (d) AAU and AGU Below is a segment of RNA from the middle of an mRNA 7-42 5'-UAGUCUAGGCACUGA-3 ' If you were told that this segment of RNA was part of the coding region of an mRNA for large protein, give the amino acid sequence for...
Please help with 4-10! DNA, Genes,and Protein Synthesis Activity 13: 2. The bases that interact with each other are called complementary bases. this definition and your answers to 1 complete th...
Please help with 4-10!
DNA, Genes,and Protein Synthesis Activity 13: 2. The bases that interact with each other are called complementary bases. this definition and your answers to 1 complete the following: a. Thiamine (T) is the complementary base of b. Cytosine (C) is the complementary base of c. Adenine (A) is the complementary base of d. Guanine (G) is the complementary base of Based on 3. Shown below is the nucleotide sequence for one strand of a stretch of...
expressed at high levels, comparable to its expression in the absence of trp 6. Based upon...
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10. When considering regulation of gene expression A. miRNA/RISC complex binding will lead to degradation of target mRNA. B. siRNA/RISC complex binding will halt translation of the target mRNA. C. proteins are destroyed by the proteasome using GTP energy D. labeling of proteins for destruction by the proteasome requires ATP energy 11. When comparing the epigenomes of normal and cancerous breast epithelial cells A. cancerous cells increase the methylation of genes that promote cell growth. B. cancerous cells increase...
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Genetics Worksheet Week 3: Gene Regulation and Epigenetics 1. Duchenne muscular dystrophy is caused by a mutation in a gene that is 2.5 million nucleotides in length and encodes a protein called dystrophin. The dystrophin protein itself is 3684 amino acids in length. Calculate below the approximate size of the mRNA that encodes dystrophin. Approximately what percentage of the gene that encodes dystrophin is intron sequence? The human genome encodes a much greater variety and number of proteins than the...
someone please help! i have a brain fart :/ tations and Regulation of Gene Expression 6....
someone please help! i have a brain fart :/
tations and Regulation of Gene Expression 6. Which of the following statements about the DNA in your brain cells is TRUE? a. The majority of the DNA in these cells is made up of genes that code for proteins. b. The majority of genes in these cells are expressed as RNA or proteins. C. Your brain cells contain approximately the same DNA as your heart cells. d. All of the above...
Please note that Questions 15 to 17 are connected questions. Question 15: The following shows a...
Please note that Questions 15 to 17 are connected
questions.
Question 15:
The following shows a partial DNA sequence from the wild-type
(normal) allele for the human leukemia-linked apoptotic
gene.
5' ATGCGATTAATCGGTAAA 3' (non-template strand)
3' TACGCTAATTAGCCATTT 5' (template strand)
Please answer the following questions:
(a) If the bottom strand serves as the DNA template for
transcription, what is the resulting mRNA sequence?
The mRNA sequence is 5' 3'. (2
marks)
5' AUG CGA UUA AUC GGU AAA 3' ?
Please enter...
Page < 2 > of 2 D. The trp operon would be expressed at high levels,...
Page < 2 > of 2 D. The trp operon would be expressed at high levels, comparable to its expression in the absence of trp 6. Based upon what you have learned about the E. coli trp operon, what could you say about expression of its structural genes in cells with large amounts of environmental trp, but expressing a mutant trp repressor that is unable to bind trp? A. The trp operon would be completely repressed due to the abundance...
ASSIGNMENT For the DNA sequence given below, write the complementary DNA sequence that would complete the...
ASSIGNMENT For the DNA sequence given below, write the complementary DNA sequence that would complete the double-strand. DNA 3’—A T T G C T T A C T T G C A T -- 5’ DNA 5’-- Does it matter which strand is the ‘code strand’? The following two sequences look identical, except one runs 3’-5’ and the other 5’-3’. For each DNA sequence given below, write the mRNA sequence that would be coded from it. Make sure you indicate the direction of each mRNA strand (i.e. 3’ and 5’ ends). Use the Universal triplet code to...
1 2 3 4 5 lane lane (fig60) -- Lin-41 western blot let-7 northern blot The images above show a northern blot using a probe to identify the let-7 miRNA (left), and a western blot (right) with a primary antibody to recognize Lin-41, a let-7 target. Assume each lane in both the northern and western blots come from the same sample. Which lane best matches each scenario described listed below? Normally processed let-7 miRNA let-7 miRNA in a Drosha mutant...
7-41 Which of the following pairs of codons might you expect to be read by the same tRNA as a result of wobble? (a) (b) CUU and UUU GAU and GAA CAC and CAU (d) AAU and AGU Below is a segment of RNA from the middle of an mRNA 7-42 5'-UAGUCUAGGCACUGA-3 ' If you were told that this segment of RNA was part of the coding region of an mRNA for large protein, give the amino acid sequence for...
Please help with 4-10!
DNA, Genes,and Protein Synthesis Activity 13: 2. The bases that interact with each other are called complementary bases. this definition and your answers to 1 complete the following: a. Thiamine (T) is the complementary base of b. Cytosine (C) is the complementary base of c. Adenine (A) is the complementary base of d. Guanine (G) is the complementary base of Based on 3. Shown below is the nucleotide sequence for one strand of a stretch of...
expressed at high levels, comparable to its expression in the absence of trp 6. Based upon what you have learned about the E. coli trp operon, what could you say about expression of its structural genes in cells with large amounts of environmental trp, but expressing a mutant trp repressor that is unable to bind trp? A. The trp operon would be completely repressed due to the abundance of trp B. The trp operon would be transcribed much less than...
10.
10. When considering regulation of gene expression A. miRNA/RISC complex binding will lead to degradation of target mRNA. B. siRNA/RISC complex binding will halt translation of the target mRNA. C. proteins are destroyed by the proteasome using GTP energy D. labeling of proteins for destruction by the proteasome requires ATP energy 11. When comparing the epigenomes of normal and cancerous breast epithelial cells A. cancerous cells increase the methylation of genes that promote cell growth. B. cancerous cells increase...
Genetics Worksheet Week 3: Gene Regulation and Epigenetics 1. Duchenne muscular dystrophy is caused by a mutation in a gene that is 2.5 million nucleotides in length and encodes a protein called dystrophin. The dystrophin protein itself is 3684 amino acids in length. Calculate below the approximate size of the mRNA that encodes dystrophin. Approximately what percentage of the gene that encodes dystrophin is intron sequence? The human genome encodes a much greater variety and number of proteins than the...
someone please help! i have a brain fart :/
tations and Regulation of Gene Expression 6. Which of the following statements about the DNA in your brain cells is TRUE? a. The majority of the DNA in these cells is made up of genes that code for proteins. b. The majority of genes in these cells are expressed as RNA or proteins. C. Your brain cells contain approximately the same DNA as your heart cells. d. All of the above...
Please note that Questions 15 to 17 are connected
questions.
Question 15:
The following shows a partial DNA sequence from the wild-type
(normal) allele for the human leukemia-linked apoptotic
gene.
5' ATGCGATTAATCGGTAAA 3' (non-template strand)
3' TACGCTAATTAGCCATTT 5' (template strand)
Please answer the following questions:
(a) If the bottom strand serves as the DNA template for
transcription, what is the resulting mRNA sequence?
The mRNA sequence is 5' 3'. (2
marks)
5' AUG CGA UUA AUC GGU AAA 3' ?
Please enter...
Page < 2 > of 2 D. The trp operon would be expressed at high levels, comparable to its expression in the absence of trp 6. Based upon what you have learned about the E. coli trp operon, what could you say about expression of its structural genes in cells with large amounts of environmental trp, but expressing a mutant trp repressor that is unable to bind trp? A. The trp operon would be completely repressed due to the abundance...
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