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Of the following steps to make recombinant DNA, which step occurs immediately after a restriction enzyme...

Of the following steps to make recombinant DNA, which step occurs immediately after a restriction enzyme recognizes specific nucleotide sequences?

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Answer #1

after the restriction enzyme recognise the restriction site

  • cleavage of the restriction site occur
  • sticky ends are formed
  • annealing of the gene of insert to the site where restriction enzyme made a cut.
  • dna ligase is required to seal the gap.
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