ANS) qPCR that is quantitative polymerase chain reaction is a sensitive technique that helps in determining protein to protein interaction because they work on real time basis. The qPCR illuminates each protein molecules with a different wavelength to study their interactions.
Can you determine protein-protein interation from qPCR? If so, please explain how.
Assume that you are using qPCR to determine the amount of DNA in a given sample. Sample 1, from which you know that it contained an amount of 1 pg, required 15 PCR cycles to reach a specific level of PCR product. Your sample 2 required only 5 PCR cycles to reach the same level. Sample 3 required 18 PCR cycles to come to the same level of PCR product. How much DNA did samples 2 and 3 contain? RT-qPCR...
explain how you can guess if a specific protein is a transmembrane protein.
Please explain in detail how to determine the answer : If a part of a protein has sequence of GLEDKSA and forms one continuous α-helix, which amino acid is the carbonyl of glycine non-covalently boned to?
The answer is C, can someone please explain why this is the
answer?
You purify bee (Apis mellifera). After a year of research, you determine that the enzyme is essential for a pathway that allows the organism to produce venom. You are now interested in how the enzyme is regulated, so you conduct a series of experiments to help you address this aim. You finally determine that the enzyme is inhibited under conditions that prevent its dephosphorylation. What do these...
Please explain and show all steps so I can study from it. You measure the elastic modulus of a stack of pancakes and determine it to be 20000 Pa. If you assume the interaction energy between the glutenin molecules is Uint=100kBT=4×10-19J, what is the characteristic bond length? Please report your answer in nanometers (nm). Recall there are 1,000,000,000 nm in one meter. to solve this problem, you need to use the Equation of the Week: E= Uint ------ l3
Please explain how to do this so I can complete the other
questions - Thank you!
Express your answer as an integer. PO ALQ O a ? 3.42 pm Submit Request Answer
Problem 10 (3 points): Assume that you are using qPCR to determine the amount of DNA in a given sample. Your calibration sample, which contained an amount of 10-15 mol, required 12 PCR cycles to reach a specific level of PCR product. Your cell sample with an unknown DNA amount required only 9 PCR cycles to reach the same level. How many moles of DNA did the cell sample contain? Describe how you arrived at that conclusion.
Explain how Niacin can be used to reduce a ketone. Please provide a mechanism. Draw by hand Explain how a cell ubiquitinates a protein and provide two possible reasonings for doing so. Explain how high levels of amino acids can act as feedback inhibitors.
Before you perform any protein assays on an sample of unknown protein concentration, how can you figure out the protein concentration range? Presumably, Bradford and Lowry assays work on certain protein ranges. If you had a sample of unknown concentration how would you determine its concentration range? Step by step if you could
You use the Bradford (coomassie) assay to determine the concentration of Protein A. You use bovine albumin as your standard. You determine the Protein A concentration to be 2 mg/ml. When you use Protein A as your standard, you determine the Protein A concentation of your unknown to be 3 mg/ml. Explain the discrepancy (pick ONE answer): (2 points) a. Protein A has fewer basic amino acids than albumin. b. Protein A has more acidic amino acids than albumin c....