What methods are available for testing the bioactivity of the gdhA gene? For example, for the...
What methods are available for testing the bioactivity of the gdhA gene? For example, for the gdhA assay, we will use of the biochemical property of the expressed enzyme that it is NADP-specific glutamate dehydrogenase. To test bioactivity, we use a spectrophotometer. What other methods can be used?
Homework Answers
flourescent methods : using flouresent coated substrate for the glutamate dehydrogenase enzyme can identify the bioactivity based on flourescent levels
Chemiluminescent methods : using a substrate to the glutamate dehydrogenase enzyme that gets converted into ia product that emits luminescence , we can measure the bioactivity of enzyme using the chemiluminescence level
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What methods are available for testing the bioactivity of the
gdhA gene? For example, for the...
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i cant actually find out, so helping me out finding an example ans filling out these...
i cant actually find out, so helping me out finding an example
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Number 5
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i cant actually find out, so helping me out finding an example
ans filling out these questions would be highly appriciated.
9. Look up one of the commercially available ELISA assay on the web. Name of the company and the assay, What molecules is the ELISA detecting? What is the sensitivity of the assay in the biochemical sense? (What is the smallest concentration or amount that can be detected?) What is this ELISA used for?
Number 5
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