Question

. You encounter a mutant line of cells which is consistently producing incorrectly folding versions of proteins. You isolate many copies of one specific protein and compare it to the same one made in a healthy cell. Below are these protein samples. Give a possible explanation for your observations. Be as specific as possible.

Healthy cell’s protein:            A H D W K L D D E C W V C L L W C W A L V I W I W H H R

                                   

Mutant cells’ proteins:            A H D V K L D D E C V V C L L V C W A L V I V I W H H R

                        A H D V K L D D E C V V C L L W C V A L V I V I W H H R

                        A H D V K L D D E C V V C L L V C W A L V I V I V H H R

                        A H D W K L D D E C W V C L L V C V A L V I W I W H H R

                        A H D V K L D D E C V V C L L W C V A L V I W I V H H R

Answer 1

Answer

Healthy cell’s protein:            A H D W K L D D E C W V C L L W C W A L V I W I W H H R

                                   

Mutant cells’ proteins: A H D V K L D D E C V V C L L V C W A L V I V I W H H R

A H D V K L D D E C V V C L L W C V A L V I V I W H H R

A H D V K L D D E C V V C L L V C W A L V I V I V H H R

A H D W K L D D E C W V C L L V C V A L V I W I W H H R

A H D V K L D D E C V V C L L W C V A L V I W I V H H R

In all the mutant cases there is specific mutation where Tryptophan is replaced by valine amino acid in all cases [bolded letter]. Tryptophan has high molecular weight and this result in hydrophobic interaction, so these all contributed to non functional protein