How can you determine whether glucose added to a suspension of isolated mitochondria is metabolized to...
How can you determine whether glucose added to a
suspension of isolated mitochondria is metabolized to CO2 and
H2O?
Homework Answers
For this you have to add something which can differentiate themselves when they are releasing. For this you can add radioactive elements in the initial substrate.
Experiment 1- you can add radioactive glucose having radioactive carbon- 14 . And look for Carbon dioxide evolved. You will found that CO2 evolved during carbon dioxide is radioactively bound which will helps in determination of carbon dioxide during mitochondrial respiration. If radioactive CO2 is released, it means glucose release this CO2.
Experiment 2- For water determination, you should use radioactive oxygen -26 in glucose and then look after respiration. Whether radioactive oxygen is evolved or not. If radioactive H2O is evolved means glucose release Water
Experiment 3- Use both radioactive element in one glucose sugar. Then find results. In this way, you can find whether glucose is responsible for H2O and CO2 emission or not. If both have radioactive nature then it means glucose take participate in releasing H2O and CO2.
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