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you construct an E.coli strain that carries mutation in the genome that impact lactose utilization. describe...

you construct an E.coli strain that carries mutation in the genome that impact lactose utilization. describe what would happen if you were to use an E.coli strain that has a mutation in the binding site for CAP so that CAP will no longer bind. Explain how it will differ from the normal wild type E.coli

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Answer #1

When glucose is present in the cell, cAMP is not synthesized. Glucose inhibits the enzyme adenylated cyclase which converts ATP into cAMP. cAMP binds to CAP and forms a complex that binds to the promoter of the lac operon. cAMP-CAP complex helps RNA polymerase in its promoter binding.

When cell utilized all its glucose, cAMP is synthesized and lac operon is activated.

If there is a mutation in the CAP such that it cannot bind to the promoter, RNA polymerase will not be able to bind lac promoter and lac operon will be in repressed condition even in the absence of glucose and presence of lactose.

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