To start this answer, I would like to remind about the grouping of bacteria based on Gram staining. Bacteria are classified as Gram+ and Gram-ve based on their ability to retain Gram stain. Those who retain the colour are Gram+ and those who do not are Gram- . Gram +ve have thick peptidoglycan wall, and Gram -ve have thin wall.
M. smegmatis is a Gram positive bacterium.and so due to thick wall it absorbs the surrounding toxin. And E.coli is a Gram -ve bacterium, (thin peptidoglycan wall), has less ability to hold the toxin and so it ejects it.
Conclusion: The toxin will not be toxic to the E.coli.
if you discover a protein that is toxic to M. smegmatis (mycobacterium smegmatis), would this same...
2. You are trying to design a protein that will be expressed
in Escherichia coli and secreted outside of the cell for
purification and use as a pharmaceutical. E. coli is a
gram-negative cell and the protein folds after it has exited the
cell. Which secretion system would work best for this project?
Support your answer with evidence based on the properties of E.
coli, the protein, and the secretion system. (20 pts)
2. You are trying to design a...
MICROBIOLOGY. PLEASE ANSWER BOTH.
Thank you
3. You would be wasting your time staining for acid fast for which of the bacteria listed below A Bacillus licheniformis B. Mycobacterium smegmatis C. Pseudomonas aeruginosa D. ALL of the above E. Both A and C _You have two bacterial cultures (#1) has mostly endospore that have not been released from the bacteria and (#2) has all free endospores. Which would be the younger of the two cultures A. #1 B. #2 C....
You are trying to design a protein that will be expressed in Escherichia coli and secreted outside of the cell for purification and use as a pharmaceutical. E. coli is a gram-negative cell and the protein folds after it has exited the cell. Which secretion system would work best for this project? Support your answer with evidence based on the properties of E. coli, the protein, and the secretion system
You are trying to design a protein that will be expressed in Escherichia coli and secreted outside of the cell for purification and use as a pharmaceutical. E,coli is a gram-negative cell and the protein folds after it has existed the cell. Which secretion system would work best for this project? Support your answer with evidence based on the properties of E.coli, the protein , and the secretion system.
"Three organisms were innoculated on a culture media. Results showed Escherichia coli as red colonies, no growth of Staphylococcus epidermidis, and colorless colonies of Salmonella enterica. The medium is:" a selective and differential b differential c selective d minimal e heterotrophic "A bacterium was incubated with aeration in a nutrient medium containing two carbon sources. After logarithmic growth, a lag phase was observed for 25 minutes. Then logarithmic growth at a slower rate was observed. Which substate was preferred by...
How would using the SEM provide information that can help you with identifying which organism is causing a disease? Would you be able to tell the difference between two different rod-shaped bacteria (say E. coli and Salmonella) using SEM? Or is there another type of microscope that could help you do that?
You want to make E. coli bacteria that glow green. Your plan is to insert the gene for green fluorescent protein (GFP) from a jellyfish (a eukaryote) into the genome of the bacteria so that the mRNA is transcribed by the E. coli cells and translated into protein. What changes do you need to make to the jellyfish GFP gene sequence to make sure that the bacteria will make a functional protein? Why? (1 point) A type of amatoxin binds...
lake-up Micro Lab: Identifying Unknown bacteria: dentify the Unknown Bacteria Lab Experiment elow are results from the unknown lab experiment. The identity of this bacteria is not known. Your ask is to use the data below to form a hypothesis of the identity of this bacteria. Record your results on he chart (Table 1) below and make a prediction on the bacteria based on your results. There is a list of ossible bacteria below. You may need to look back...
1) Imagine that you are interested in studying the enzyme dihydrofolate reductase (DHFR) from Mycobacterium tuberculosis (this enzyme is a potential drug target). The amino acid sequence of the protein is: MTMVGCIWAQATSGVIGRGGDIPWRLPEDQAHFREITMGHTIVMGRRTWDSLPAKVRPLPGRRNWL SRQADFMASGAEWGSLEEALTSPETWVIGGGQVYALALPYATRCEVTEVDIGLPREAGDALAPVLD ETWRGETGEWRFSRSGLRYRLYSYHRS The DNA sequence within the M. tuberculosis genome that codes for the protein is: ATGACGATGGTGGGGCTGATCTGGGCTCAAGCGACATCGGGTGTCATCGGCCGCGGCGGCGACATCCCCT GGCGCTTGCCCGAGGACCAGGCGCATTTCCGGGAGATCACCATGGGGCACACGATCGTGATGGGCCGGCG CACATGGGATTCGCTGCCGGCTAAAGTCCGGCCGCTGCCCGGCCGGCGAAATGTCGTACTGAGCCGCCAA GCTGACTTTATGGCCAGCGGGGCTGAGGTTGTCGGTTCACTCGAGGAGGCGCTGACCAGCCCGGAGACGT GGGTGATCGGAGGCGGACAAGTCTATGCGCTGGCGCTGCCGTACGCGACCAGATGTGAGGTTACCGAGGT CGACATCGGCCTGCCGCGCGAAGCCGGTGACGCGCTGGCCCCCGTGCTGGACGAGACATGGCGGGGCGAG ACGGGGGAGTGGCGCTTCAGCCGGTCCGGGTTGCGGTACCGGTTGTACAGCTACCACCGCTCATGA Assume that you have been given a sample of the bacterial genomic DNA, restriction enzymes Ndel and BamHi, and a plasmid from Novagen called PET-28b....
Consider an important protein like beta-galactosidase, which breaks down lactose. Many bacterial species have beta-galactosidase. But that doesn't mean that the amino acid sequence of beta- galactosidase in all those bacterial species is exactly the same. Far from it. As bacteria diversified into different species over evolutionary time, beta-galactosidase encoded in the genomes of the bacteria also diversified. However, we can assume that even though beta-galactosidase diversified, it was under pressure to maintain structure and function, because the reaction it...