Question

Methods and material for the experiment of effect of enzyme concentration. Explain briefly.

Answer 1

Materials:  

Different labelled Test tube, different enzyme concentration sample , a particular substrate which is chromogenic , incubator, phosphate buffer solution for stabilising enzyme. Stop watch, spectrophotometer, pippete

Methods:

In this you will add all the materials in different test tubes.

Don't forget to add substrate concentration.

Then add enzyme concentration in increasing order from one test tube to others.

Then put these test tubes in incubator so that reaction will occur and look your product on the basis of colour at different time intervals.

Then look for the result, by means of colour or spectrophotometer absorbance readings, you will find that tubes which has higher concentration of enzyme in test tube, substrate will convert into product faster as compared to lower concentration of enzyme containing test tube.