1. If the egfp gene is approximately 700 bp in length, why do we predict the...
1. If the egfp gene is approximately 700 bp in length, why do we predict the PCR product from positive clones in Lab Session 8B to be approximately 1200bp in length?
2. Is it possible for a clone to have multiple copies of egfp inserted? Could two copes be inserted? Could three copes be inserted? If so, what would be the result of the PCR screen?
Homework Answers
Q.1 egfp gene is 700 bp in length but we amplify entire DNA segment in PCR which contains inserted gene at multiple cloning site too. SO the ned product will have egfp gene along with desired gene added at MCS
Q.2 it is possible to clone another egfp gene at multiple cloning site but it will have no additional effect in end product apart from increase in size.
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