Which restriction enzyme could NOT cleave the following sequence: 5’-AATATAGGTACCTCGAGGGCCCTGAT-3’ BstBI ApaI KpnI XhoI
- Which restriction enzyme could NOT cleave the following sequence:
5’-AATATAGGTACCTCGAGGGCCCTGAT-3’
- BstBI
- ApaI
- KpnI
- XhoI
Homework Answers
The answer is A. BstBI as it recognizes TT^CGAA sites which is not present in given sequence.
ApaI recognizes GGGCC^C sites.
KpnI recognizes GGTAC^C sites .
XhoI recognizes C^TCGAG sites.
Add Answer to:
Which restriction enzyme could NOT cleave the following
sequence:
5’-AATATAGGTACCTCGAGGGCCCTGAT-3’
BstBI
ApaI
KpnI
XhoI
Question #4 (5 pts): Select a restriction enzyme, determine its cleavage sites, which organism is was derived from and how this enzyme doesn't cleave in the organism it is derived (minus poin...
Question #4 (5 pts): Select a restriction enzyme, determine its cleavage sites, which organism is was derived from and how this enzyme doesn't cleave in the organism it is derived (minus points if you choose enzymes found in the text)?
Question #4 (5 pts): Select a restriction enzyme, determine its cleavage sites, which organism is was derived from and how this enzyme doesn't cleave in the organism it is derived (minus points if you choose enzymes found in the text)?
The table shows where different restriction endonucleases (restriction enzymes) cleave DNA. The abbreviation R represents the...
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Question #4 (5 pts): Select a restriction enzyme, determine its cleavage sites, which organism is was derived from and how this enzyme doesn't cleave in the organism it is derived (minus points if you choose enzymes found in the text)?
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Question #4 (5 pts): Select a restriction enzyme, determine its cleavage sites, which organism is was derived from and how this enzyme doesn't cleave in the organism it is derived (minus points if you choose enzymes found in the text)?
Question #4 (5 pts): Select a restriction enzyme, determine its cleavage sites, which organism is was derived from and how this enzyme doesn't cleave in the organism it is derived (minus points if you choose enzymes found in the text)?
The table shows where different restriction endonucleases (restriction enzymes) cleave DNA. The abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation W represents adenine or thymine. Enzyme EcoRI EcoRV Target sequence 5' GAATTC 3 3' CTTAAG 5 5' GATATC 3 3' CTATAG 5 5' GGCC 3' 3' CCGG 5 5' AAGCTT 3 3' TTCGAA 5 5' RGGWCCY 3 3' YCCWGGR 5 Cleavage 5G AATTC 3' 3' CTTAA G 5'...
3.13 pts) The restriction enzyme known as Notl recognizes the following sequence: 5-GCGGCCGC-3 3-CGCCGGCG-5 However, if the cytosines in this sequence have been methylated. NotI will not cleave the DNA at this site. For this reason, Net is commonly used to investigate the methylation state of CpG islands. A researcher has studied a gene, which we will call T. that is found in com, and encodes a transporter involved in the uptake of phosphate from the soil. A CpG island...
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The table shows where different restriction endonucleases (restriction enzymes) cleave DNA. The abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation W represents adenine or thymine. Enzyme EcoRI EcoRV Target sequence 5' GAATTC 3' 3' CTTAAG 5 5' GATATC 3 3' CTATAG 5 5' GGCC 3 3' CCGG 5 5' AAGCTT 3' 3' TTCGAA 5 5'RGGWCCY 3 3' YCCWGGR 5 Cleavage 5'G AATTC 3'...
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