3. Assume presence of galactose and absence of glucose, and that mutants are loss of function....
3. Assume presence of galactose and absence of glucose, and that mutants are loss of function. In a Gal 80 mutant, gal genes will/will not _________________ be expressed. In a gal4 mutant, gal genes will/will not ________________ be expressed. In a gal80/gal4 double mutant, the gal genes will/will not _________________ be expressed. If a scientist replaced the activation domain of gal4 with a histone deactylase domain, the gal genes will/will not ______________ be expressed.
Homework Answers
Answer
Activity of GAL4 operon is regulated by multiple proteins. Gal80 (repressor) interacts with gal4 (activator) and inhibits the action of gal4 and hence decreases the expression of gal operon.
In a Gal 80 mutant, gal genes will be expressed. In a gal4 mutant, gal genes will not be expressed. In a gal80/gal4 double mutant, the gal genes will not be expressed. If a scientist replaced the activation domain of gal4 with a histone deactylase domain, the gal genes will be expressed.
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3. Assume presence of galactose and absence of
glucose, and that mutants are loss of function....
Answer all questions and explain in detail. 1) GAL80 loss-of-function mutants express GAL1, 2, 7, 10,...
Answer all questions and explain in detail. 1) GAL80 loss-of-function mutants express GAL1, 2, 7, 10, genes constitutively (in the absence of galactose) because a transcriptional activation by GAL4 is not inhibited b GAL80 is not present to block GAL4 DNA-binding c GAL80 cannot respond to GAL3 signaling d A and B e B and C 2) Gal80-; GAL4- double mutants express the GAL1, 7, 10 genes a only In the presence of galactose b only in the absence of...
GAL80 loss-of-function mutants express the GAL1, 2, 7, 10 genes constitutively in the absence of galactose)...
GAL80 loss-of-function mutants express the GAL1, 2, 7, 10 genes constitutively in the absence of galactose) because A. transcriptional activation by GAL4 is not inhibited B. GAL80 is not present to block GAL4 DNA-binding C. GAL80 cannot respond to GAL3 signaling D. A and B E. B and C TB 68
The yeast Saccharomyces cerevisiae can grow as haploid or diploid cells. You have two haploid yeast...
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GAL80 loss-of-function mutants express the GAL1, 2, 7, 10 genes constitutively in the absence of galactose) because A. transcriptional activation by GAL4 is not inhibited B. GAL80 is not present to block GAL4 DNA-binding C. GAL80 cannot respond to GAL3 signaling D. A and B E. B and C TB 68
Genetics Worksheet Week 3: Gene Regulation and Epigenetics 1. Duchenne muscular dystrophy is caused by a mutation in a gene that is 2.5 million nucleotides in length and encodes a protein called dystrophin. The dystrophin protein itself is 3684 amino acids in length. Calculate below the approximate size of the mRNA that encodes dystrophin. Approximately what percentage of the gene that encodes dystrophin is intron sequence? The human genome encodes a much greater variety and number of proteins than the...
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