Promoters are cis-acting. The promoter is considered a cis-acting site because:
A it influences the expression of a family of genes within a protein domain.
B it exclusively influences the expression of genes to which the promoter is proximal.
C it activates the gene it is proximal to while inhibiting the expression of all others.
D it regulates the transcription of both alleles of an allele pair.
E promoters bind to the sigma subunit.
B it exclusively influences the expression of genes to which the promoter is proximal.
Promoters are cis-acting. The promoter is considered a cis-acting site because: A it influences the expression...
Compare and contrast the role of promoters versus enhancers in controlling gene expression by answering the following questions. Are they cis- or trans- acting components of the regulatory machinery? Explain why this label applies to each? Where are they located relative to the transcription start site (TSS) of a gene? What kinds of transcription factors (TFs) does each element bind, and what are the particular TFs’ role in gene expression?
1. trans-acting factors are able to regulate target genes from any chromosome, whereas cis-acting elements can only regulate genes located in the same chromosome. a. True only in operons. In Eukaryotic systems, trans-acting factors only regulate genes in the same chromosome. b. True only in Eukaryotes. Prokaryotes don’t have cis-acting elements. c. True for any organism. d. False. The statement is erroneous 2. Unlike activators, repressors never affect chromatin structure. Repressors inhibit transcription only by binding to the binding sites...
3. Prokaryotic and eukaryotic gene expression compared. Below is an incomplete table of prokaryotic and eukaryotic gene expression in comparison. Fill in the blank using PPT slides, notes and the textbook. Prokaryotic gene expression Eukaryotic gene expression Overview Steps Transcription and translation Yes Transcription and translation coupled? Gene structure No introns Epigenetic modification (chromosome remodeling) transcription, translation, RNA processing, protein processing Transcription in the nucleus and translation in the cytoplasm Interrupted gene with exons and introns RNAPI, II, III Which...
Answer the questions:
Question 11 Recognition/binding site of RNA polymerase is called a Receptorb. Promoter . Facilitatord. Terminator Question 12 .A specific factor helps RNA polymerase binding to promoters and transcribe genes a Delta b. Beta Gamma d. Sigma Question 13 ............ Promoters lack a TATA box are referred to as TATA less promoters, for example operon Housekeeping genes b. Functional genesc d. Structural genes Question 14 0.5 points Save Answer During "RNA processing" All of the exons are a....
The lac operon contains a DNA sequence known as the lac promoter (P or P+ for wild type; P– for mutant (RNA polymerase does not bind)) that serves as the RNA polymerase binding site. The lac operon also contains a DNA sequence known as the Lac operator (O or O+ for wild type; O– or Oc for mutant (lac repressor cannot bind)) which is the binding site for lac repressor. The lac repressor, a protein, is encoded by the lac...
Question 1 Match the term with the best definition or description; most topics relate to the regulation of gene expression. General type of protein which will increase transcription rates when it attaches to a site A. Factor connected to a particular gene - B. Co-repressor C. Enhancer D. Promoter E. Structural F. Intron G. Activator H. Operator I. Basal transcription J. Glucocorticoid receptor K. Sigma factor L. Mediator M. Inducer N. TATA box O. Repressor The rates of mRNA produced...
A genetically engineered plasmid Ficarries all of the elements of the lac operon, but replaces the structural genes with the reporter gene green fluorescent protein (GFP). The repressor allele cannot bind lactose, and the OC allele cannot be bound by the repressor. Given the following genotypes and growth media that does not contain glucose, indicate in the table below whether you expect to see b-galactosidase activity and glowing green colonies of E. coli by selecting Yes or No. growth media...