List cis regulatory regions and transacting factors in lac operon?
List cis regulatory regions and transacting factors in lac operon?
Homework Answers
Cis regulatory regions are those which regulate the gene located nearby to it. In case of Lac Operon operator is a cis regulatory region. It codes for lactose repressor which binds to promoter and repress transcription of Lac Operon.
Transacting factors are those which have been transcribed from a region which is located very far from the gene which it controls. In case of Lac Operon, transcription factors that bind to promoter to initiate transcription of Lac Operon are trans acting elements. They proteinaceous molecules which have been transcribed from some other far located gene but regulate Lac Operon.
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Describe/draw the lac operon (including both the regulatory regions and the coding regions) and explain how...
Describe/draw the lac operon (including both the regulatory regions and the coding regions) and explain how its expression is regulated. Is it inducible or repressible? Explain why you answered this way.
Describe and label the regions on the lac operon. Include the following: regulatory gene, promoter region,...
Describe and label the regions on the lac operon. Include the following: regulatory gene, promoter region, operator, structural genes, and repressor. What happens in the presence of glucose? Lactose?
A bacterial inducible operon, similar to the lac operon, contains three regions (R,T and S) that...
A bacterial inducible operon, similar to the lac operon, contains three regions (R,T and S) that are involved in coordinated regulation of transcription . One of these regions is the operator, one is a gene for a regulatory protein and the third is a gene that produces an enzyme. In the table below,
18. Draw the lac operon with all cis and trans acting factors A. in the presence...
18. Draw the lac operon with all cis and trans acting factors A. in the presence of lactose B. in the presence of lactose plus glucose C. in the presence of lactose plus glucose in a OC mutant
The Lac operon is a classic example of regulatory logic. Summarize the details of the control...
The Lac operon is a classic example of regulatory logic. Summarize the details of the control of the Lac operon pathway, and also explain how this is advantageous for the organism as a whole.
What are “cis-regulatory elements,” “trans-regulatory factors,” “exons,” “introns,” “untranslated regions,” “proximal promoters,” “enhancers,” “repressors,” "activators,” “caps,”...
What are “cis-regulatory elements,” “trans-regulatory factors,” “exons,” “introns,” “untranslated regions,” “proximal promoters,” “enhancers,” “repressors,” "activators,” “caps,” and “poly-A tails”? Which are protein coding sequence (or are actual proteins); which are not? Which make it into mature mRNA?
Catabolite repression can regulate the expression of the lac operon in E. coli. In terms of...
Catabolite repression can regulate the expression of the lac operon in E. coli. In terms of this positive regulation of the lac operon: What is the activator? Would it work in cis or trans? What molecule binds to the allosteric domain of the activator? What influences strongly the levels of the molecule you answered for part b? To what regulatory sequence does the activator bind? What part/domain of the activator? What is the effect of this binding? Name the two...
Lac Promoter i promoter galactoside transacetylase gene E coll chromosome lac operon loc terminator regulatory gene...
Lac Promoter i promoter galactoside transacetylase gene E coll chromosome lac operon loc terminator regulatory gene Operator site (laco) CAP site Lactose permease gene Beta-galactosidase gene
Briefly explain your answers to the following questions. A mutation (Mutation A) in the lac operon...
Briefly explain your answers to the following questions. A mutation (Mutation A) in the lac operon of E. coli leads to an inability to ferment lactose, and the expression of the operon is always off. Introduction into the mutant of an F' factor containing the wild type lac operon does NOT restore the ability to ferment lactose, i.e., there is no expression of the operon from the plasmid either. a. What is the probable nature of Mutation A? Is the...
1) The lac operon is… a) Positive repressible b) Both negative inducible and positive repressible c)...
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Lac Promoter i promoter galactoside transacetylase gene E coll chromosome lac operon loc terminator regulatory gene Operator site (laco) CAP site Lactose permease gene Beta-galactosidase gene
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