Question

 Consider a standard PCR reaction.  If instead of including double-stranded DNA from

a biological sample, you added mRNA molecules from a biological sample, the reaction

would… Can you please explain why the answer is not E

A. work, but would yield less product because you started off with single-stranded

template 

B. A and D are correct 

C. work, but would generate much shorter products 

D. not work because you would also need to replace the dNTPs with NTPs for the

reaction to work 

E. not work because the enzyme will not have the type of template it needs

Answer 1

Ans cannot be option E because Taq DNA Polymerase has an intrinsic RNA-dependent DNA polymerase activity (reverse transcriptase activity). However, this activity is very low and mRNA needs to be present in large quantity.

Amount of Product will be less .