Question

The following is a fragment of double-stranded DNA. It encodes a hypothetical 6 amino acid protein and includes the start (initiator) codon, a small amount of 5' UTR, and a small amount of 3' UTR. TTGGCAATGTGATCCCTTGTGCGGTACCACT AACCGTTACACTAGGGAACACGCCATGGTGA The bottom strand is the template, and the RNA polymerase moves along the bottom (template) strand from RIGHT TO LEFT. a) Determine the orientation of the DNA template. The template strand is copied below. (Enter either 5' or 3' in the box below, be sure to include the apostrophe) - AACCGITACACTAGGGAACACGCCATGGTGA b) in the text box below, write out the sequence and orientation of the RNA that is transcribed from this template Identify the S' and 3'ends, and start with the s'end. (Format your answer exactly as follows: S-XXXXX.XXXXXX_3" Be sure to include the apostrophes and the dashes before and after the actual sequence: NO SPACES. c)Now, use the genetic code to determine the amino acid sequence encoded by this mRNA. Counting from the N-terminus, amino acid #3 is and amino acid #5 is (Enter the three-letter abbreviation for each amino acid

Answer 1

a) 5' A A C C G T T A C A C T A G G G A A C A C G C C A T G G T G A 3' - DNA TEMPLET

b) 5 'U C A C C A U G G C G U G U U G G G U A G U G U A A C G G U U 3' - CORRESPONDING RNA TEMPLET

c) - N - terminal 3rd amino acid is Cystie ( Green)

5th amino acid is Valine ( Valine )

because A U G is the starting codon for mRNA translation. and methionine is first amino acid.