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10) I was asked to perform double digestion of a given plasmid (map below) with restriction...
Now determine the sizes of the fragments generated after digesting the r-plasmid with each of the...
Now determine the sizes of the fragments generated after digesting the r-plasmid with each of the restriction enzymes or combination of restriction enzymes. r-plasmid digested with Pstl r-plasmid digested with EcoRI r-plasmid digested with both Pstl and EcoRI Question 8. (3 pts.) Use this information to determine the relative placement of these restriction sites in the r-plasmid, and then draw restriction site maps of the vector and the r-plasmid on the following circular chromosome templates. The map should show the...
B4. Answer ALL parts: The diagram below shows the restriction pattern of a plasmid cut with...
B4. Answer ALL parts: The diagram below shows the restriction pattern of a plasmid cut with the enzymes BamHI, EcoRI and Ndel. The digests are carried out with each enzyme alone and then with different combinations of the three enzymes. Enzyme Fragment sizes kb BamHI 1.4 10.6 EcoRI 4.5 7.5 Ndel 2.5 9.5 BamHI + EcoRI 45 3.4 1.4 BamHI + Ndel 1.4 2.5 5.9 EcoRI + Ndel 0.5 2.0 2.5 7.0 (a) Draw an agarose gel with restriction fragments...
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb)....
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb). Using this map as a guide, give the number of restriction fragments along with their associated lengths that would result from digesting PGEN101 with the restriction enzymes EcoRI, BamHII, anda combination of EcoRI + BamHI. BamHI BamHI BamHI / PGEN101 (20 kb) Mb EcoRI Digest Performed Size Emments Obtained EcoRI........ BamHI.. EcoRI + BamHI.... Two freshmen college students, interested in becoming gene jocks, performed...
Practice Problem 1 A restriction map for a fictitious 4000 base pair plasmid is shown in...
Practice Problem 1 A restriction map for a fictitious 4000 base pair plasmid is shown in Figure 5. Create a figure predicting the appearance of a gel showing digests with EcoRI, BamHI, and EcoRI and BamHI combined. Your figure should include a lane showing a 1kbladder for reference. (A photo of a 1kb ladder is provided in Figure 3A.) > > FOORIOL ULO BamHI (620) 325 BamHI (3090) Abel 23 Plasmid 4000bp BamHI (945) 2145 Figure 5. A restriction map...
construct a restriction of a circular pvn plasmid, using the following data yor map should indicale,...
construct a restriction of a circular pvn plasmid, using the following data yor map should indicale, either the relative positions of each restriction site or give the distances between the restriction J DNA sizes of fragments ( bp) uncut DNA 112000 PNA cut with sma I 12000 DNA cut with Hind III 2000 PNA cut with ECORI 12000 onn cut with sma I FEcori 7000, 5000 ana cut with SMO I + HINDIII 10.000, 2000 min cut with Hind II...
A restriction map lists the locations of DNA sequences that are cut by a particular restriction...
A restriction map lists the
locations of DNA sequences that are cut by a particular restriction
enzyme for a piece of DNA, such as a chromosome or a plasmid.
Restriction maps are important when generating a construct for
experimental use. Digesting the DNA sequence with the restriction
enzymes will result in fragmented DNA of predictable sizes, based
on the restriction map, that allow a researcher to analyze if his
or her construct was generated correctly when visualized using gel
electrophoresis....
Looking at the gel electrophoresis of pcDNA vector digestion with no enzyme, Xbal, or EcoRI below,...
Looking at the gel electrophoresis of pcDNA vector digestion with no enzyme, Xbal, or EcoRI below, answer the following question (#76). Remember pcDNA vector has both Xbal and EcoRI restriction enzyme sites within the multiple cloning sequence. GELA no enz Xbal Eceri ladder GEL B no enz_Xbal EceRl ladder 76. Which gel showed successful digestion (cutting) of the vector? A. Gel A B. Gel B C. Both A and B D. Neither Gel A or B E. Gel electrophoresis is...
A plasmid that is 3931 base pairs (bp) is digested with two restriction enzymes Nae I...
A plasmid that is 3931 base pairs (bp) is digested with two restriction enzymes Nae I and Nar I. The Nae I digestion produces two fragments. One is 3158 (bp) and the second is 773 bp. The NarI digestion produces two fragments. One is 3181 bp and the second is 750 bp. When the plasmid is digested with both enzymes four fragments are produced. They are 2656 bp, 525 bp, 502 bp, and 248 bp. Draw a circular map of...
34. Answer ALL parts: The diagram below shows the restriction pattern of a plasmid cut with...
34. Answer ALL parts: The diagram below shows the restriction pattern of a plasmid cut with the enzymes Smal, Bglil and Aval. The digests are carried out with each enzyme alone and then with different combinations of the three enzymes. Enzyme Fragment sizes kb Smal 5.9 4.3 BgII 8.2 2.0 Aval 5.3 4.9 Smal+ BglII 5.4 2.8 0.5 Smal+ Aval 3.3 2.6 2.3 2.0 Smal+-Bg!!! 5.3 2.1 2.0 0.8 (a) Draw an agarose gel with restriction fragments from the data...
Chromosomal and plasmid DNA can be cut into manageable pieces by restriction enzymes. Using agarose gel...
Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
Now determine the sizes of the fragments generated after digesting the r-plasmid with each of the restriction enzymes or combination of restriction enzymes. r-plasmid digested with Pstl r-plasmid digested with EcoRI r-plasmid digested with both Pstl and EcoRI Question 8. (3 pts.) Use this information to determine the relative placement of these restriction sites in the r-plasmid, and then draw restriction site maps of the vector and the r-plasmid on the following circular chromosome templates. The map should show the...
B4. Answer ALL parts: The diagram below shows the restriction pattern of a plasmid cut with the enzymes BamHI, EcoRI and Ndel. The digests are carried out with each enzyme alone and then with different combinations of the three enzymes. Enzyme Fragment sizes kb BamHI 1.4 10.6 EcoRI 4.5 7.5 Ndel 2.5 9.5 BamHI + EcoRI 45 3.4 1.4 BamHI + Ndel 1.4 2.5 5.9 EcoRI + Ndel 0.5 2.0 2.5 7.0 (a) Draw an agarose gel with restriction fragments...
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb). Using this map as a guide, give the number of restriction fragments along with their associated lengths that would result from digesting PGEN101 with the restriction enzymes EcoRI, BamHII, anda combination of EcoRI + BamHI. BamHI BamHI BamHI / PGEN101 (20 kb) Mb EcoRI Digest Performed Size Emments Obtained EcoRI........ BamHI.. EcoRI + BamHI.... Two freshmen college students, interested in becoming gene jocks, performed...
Practice Problem 1 A restriction map for a fictitious 4000 base pair plasmid is shown in Figure 5. Create a figure predicting the appearance of a gel showing digests with EcoRI, BamHI, and EcoRI and BamHI combined. Your figure should include a lane showing a 1kbladder for reference. (A photo of a 1kb ladder is provided in Figure 3A.) > > FOORIOL ULO BamHI (620) 325 BamHI (3090) Abel 23 Plasmid 4000bp BamHI (945) 2145 Figure 5. A restriction map...
construct a restriction of a circular pvn plasmid, using the following data yor map should indicale, either the relative positions of each restriction site or give the distances between the restriction J DNA sizes of fragments ( bp) uncut DNA 112000 PNA cut with sma I 12000 DNA cut with Hind III 2000 PNA cut with ECORI 12000 onn cut with sma I FEcori 7000, 5000 ana cut with SMO I + HINDIII 10.000, 2000 min cut with Hind II...
A restriction map lists the
locations of DNA sequences that are cut by a particular restriction
enzyme for a piece of DNA, such as a chromosome or a plasmid.
Restriction maps are important when generating a construct for
experimental use. Digesting the DNA sequence with the restriction
enzymes will result in fragmented DNA of predictable sizes, based
on the restriction map, that allow a researcher to analyze if his
or her construct was generated correctly when visualized using gel
electrophoresis....
Looking at the gel electrophoresis of pcDNA vector digestion with no enzyme, Xbal, or EcoRI below, answer the following question (#76). Remember pcDNA vector has both Xbal and EcoRI restriction enzyme sites within the multiple cloning sequence. GELA no enz Xbal Eceri ladder GEL B no enz_Xbal EceRl ladder 76. Which gel showed successful digestion (cutting) of the vector? A. Gel A B. Gel B C. Both A and B D. Neither Gel A or B E. Gel electrophoresis is...
34. Answer ALL parts: The diagram below shows the restriction pattern of a plasmid cut with the enzymes Smal, Bglil and Aval. The digests are carried out with each enzyme alone and then with different combinations of the three enzymes. Enzyme Fragment sizes kb Smal 5.9 4.3 BgII 8.2 2.0 Aval 5.3 4.9 Smal+ BglII 5.4 2.8 0.5 Smal+ Aval 3.3 2.6 2.3 2.0 Smal+-Bg!!! 5.3 2.1 2.0 0.8 (a) Draw an agarose gel with restriction fragments from the data...
Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
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