In an experiment with agarose gel eletrophoresis, the DNA samples are loaded in the wells within...
In an experiment with agarose gel eletrophoresis, the DNA samples are loaded in the wells within the gel and the electric current is turned on. When observing the samples, you notice that the DNA fragments are moving in the opposite (wrong) direction. What possible error could have caused this to occur?
Homework Answers
Solution
- Electrophoresis is the technique of separation of DNA fragments according to there size and charges across electric field.
- DNA molecule has net negative charges so they are always moves towards positive electrode.
- If we change the polarity of electrode, positive electrode at loading site then DNA fragments not moving properly in there desired direction.so they choose direction towards positive electrode.
- So this is the cause that positive electrode is present at the site of well of DNA loading site.so fragments are moves in wrong direction.
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In an experiment with agarose gel eletrophoresis, the DNA
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produced by PCR. The target fragment amplified by the primers was
1,500 bp in size.
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Refer to the picture included in question 7 for this question. Please explain how gel electrophoresis...
Refer to the picture included in question 7 for this question.
Please explain how gel electrophoresis separates the DNA fragments
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elements within your explanation (not necessarily in order of how
you should place them in your answer):
electric current and charge
size of DNA molecules
DNA samples
gel
wells
and buffer solution.
I I III I
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The picture above represents an agarose gel that was used to analyze plasmid DNA after it was cut with the restriction enzyme HindIll. The plasmid was incubated with Hindill until all of the available Hindlll cut sites were cut by HindIll. After running the sample on the gel, three bands were detected (Note that there are three wells shown at the top of the gel for loading samples, however, only the middle well was loaded with sample). Based on this...
After PCR is performed the products are run out on an agarose
gel. In the figure below, grey bands represent the wells the PCR
product was loaded into. The white bands represent DNA fragments
produced by PCR. The target fragment amplified by the primers was
1,500 bp in size.
The ladder is a standard DNA ladder containing bands of various
sizes between 5,000 and 1,000 bp. The negative control contained
only molecular grade water*. The positive control contained DNA
known...
Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
Refer to the picture included in question 7 for this question.
Please explain how gel electrophoresis separates the DNA fragments
that are produced by the Sanger technique. Include the following
elements within your explanation (not necessarily in order of how
you should place them in your answer):
electric current and charge
size of DNA molecules
DNA samples
gel
wells
and buffer solution.
I I III I
One strand of a DNA sequences is given below. Find the
EcoRI sites and indicate the cutting site with an arrow. Count the
number of bases in each fragment.
CP22: vne strand of a DNA sequence is given below. Find the EcoRI sites and indicate the cutting site with an arrow. Count the number of bases in each fragment. Restriction digest A: ATTGAATTCCGGTTAGCTTTAGAATTCCGCCATATGCGCAATTGGAATTCC Number of bases in each fragment: Now compare the same region of DNA from another individual. Where...
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