EXERCISE 17.2: Sketch an agarose gel for use in separating DNA. Make sure you label: (a) The location of the wells (b) T...
EXERCISE 17.2: Sketch an agarose gel for use in separating DNA.
Make sure you label:
(a) The location of the wells
(b) The positive electrode
(c) The negative electrode
( d) The direction of migration of the DNA in the gel
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EXERCISE 17.2: Sketch an agarose gel for use in separating DNA. Make sure you label: (a) The location of the wells (b) T...
Sketch the predicted migration pattern on an agarose gel of the following DNA samples: (1) Undigested...
Sketch the predicted migration pattern on an agarose gel of the following DNA samples: (1) Undigested pET15b plasmid; (2) pET15b digested with the EagI restriction enzyme; (3) pET15b digested with NdeI and AhdI (see pET15b vector map for location of these enzyme cutting sites). Include in your sketch a DNA base-pair ladder from 0 to 10,000 bp with 1000 bp increments. Label each band in the DNA ladder with the known size (in base-pairs), and estimate the size of the...
17. Briefly describe the process of gel electrophoresis. Include an illustration. Be sure to label the...
17. Briefly describe the process of gel electrophoresis. Include an illustration. Be sure to label the positive and negative electrodes, the wells, and to indicate the direction that the DNA will move.
The picture above represents an agarose gel that was used to analyze plasmid DNA after it...
The picture above represents an agarose gel that was used to analyze plasmid DNA after it was cut with the restriction enzyme HindIll. The plasmid was incubated with Hindill until all of the available Hindlll cut sites were cut by HindIll. After running the sample on the gel, three bands were detected (Note that there are three wells shown at the top of the gel for loading samples, however, only the middle well was loaded with sample). Based on this...
q) You make an agarose gel with ethidium bromide (EtBr) as a visualizing agent. You use...
q) You make an agarose gel with ethidium bromide (EtBr) as a visualizing agent. You use this gel to isolate DNA you want to use for a transgenic bacteria. You will purify the DNA out of the gel before inserting it into a plasmid. What might be present in the DNA? Will this cause obstacles for your experiment?
What would happen to DNA if the gel tray within an electrophoresis chamber were placed incorrectly,...
What would happen to DNA if the gel tray within an electrophoresis chamber were placed incorrectly, with the wells closest to the positive electrode? Choose one: A. DNA would remain in the gel. B. DNA bands would appear smeared. C. DNA would be pulled through the gel in the wrong direction. D. DNA would not fluoresce under UV light.
Chromosomal and plasmid DNA can be cut into manageable pieces by restriction enzymes. Using agarose gel...
Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
is a nucleus the sole source of DNA in a eukaryotic cell? WORKSHEET: EXERCISE 10 NAME:...
is
a nucleus the sole source of DNA in a eukaryotic cell?
WORKSHEET: EXERCISE 10 NAME: DATE: SECTION Twenty microliters (20 ㎕) of each sample was loaded into your gel. Convert 2OuL to milliliters (mL). I. 2. Describe the charge on the DNA molecule and specify which component of the molecule is responsible for contributing to that charge. Why do some DNA samples travel further in the gel than others? 3. 4. Once electrophoresed, how are the DNA bands visualized?...
A1. The following is the DNA sequence of a hypothetical gene for the SMALL protein. It...
A1. The following is the DNA sequence of a hypothetical gene for the SMALL protein. It is called the SMALL gene. i atgggattac actgtcacga ccaaatagcc ttcattgtat 41 caaaaggato aatcgagtta tag Imagine you are doing a research project in a laboratory and your supervisor asks you to clone the SMALL gene into the PBR322 plasmid (shown below). You must use the Pstl and EcoRI sites for your cloning. HindIII EcoRI | EcoRV BamHI 4359 0 29 185 4000 375 Sall Psti...
1) Sketch the graph of each of the following exponential functions. Make sure you label at...
1) Sketch the graph of each of the following exponential functions. Make sure you label at least three points on the graph or include a t-chart with the coordinates. a. S(x)=3 b. y=213 c. g(x)= (3) 3 d. y e. f(x) = "+1
i cant figure out how to do procedure 1. not sure if my answers are right...
i cant figure out how to do procedure 1. not sure if my
answers are right or not either.
TCG. AGCTICGA Montel-/1 DNA FINGERPRINTING-LAB REEPORT 1 23 4 56 Db L Procedure #1: a.) Look at the photo and sketch of the finished and stained paternity gel - Moodle. On the drawing to the right, sketch the location of all of the DNA bands. A2 23130 bp 9416 bp 6557 bp b.) Also on the drawing to the right, above...
17. Briefly describe the process of gel electrophoresis. Include an illustration. Be sure to label the positive and negative electrodes, the wells, and to indicate the direction that the DNA will move.
The picture above represents an agarose gel that was used to analyze plasmid DNA after it was cut with the restriction enzyme HindIll. The plasmid was incubated with Hindill until all of the available Hindlll cut sites were cut by HindIll. After running the sample on the gel, three bands were detected (Note that there are three wells shown at the top of the gel for loading samples, however, only the middle well was loaded with sample). Based on this...
Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
is
a nucleus the sole source of DNA in a eukaryotic cell?
WORKSHEET: EXERCISE 10 NAME: DATE: SECTION Twenty microliters (20 ㎕) of each sample was loaded into your gel. Convert 2OuL to milliliters (mL). I. 2. Describe the charge on the DNA molecule and specify which component of the molecule is responsible for contributing to that charge. Why do some DNA samples travel further in the gel than others? 3. 4. Once electrophoresed, how are the DNA bands visualized?...
A1. The following is the DNA sequence of a hypothetical gene for the SMALL protein. It is called the SMALL gene. i atgggattac actgtcacga ccaaatagcc ttcattgtat 41 caaaaggato aatcgagtta tag Imagine you are doing a research project in a laboratory and your supervisor asks you to clone the SMALL gene into the PBR322 plasmid (shown below). You must use the Pstl and EcoRI sites for your cloning. HindIII EcoRI | EcoRV BamHI 4359 0 29 185 4000 375 Sall Psti...
1) Sketch the graph of each of the following exponential functions. Make sure you label at least three points on the graph or include a t-chart with the coordinates. a. S(x)=3 b. y=213 c. g(x)= (3) 3 d. y e. f(x) = "+1
i cant figure out how to do procedure 1. not sure if my
answers are right or not either.
TCG. AGCTICGA Montel-/1 DNA FINGERPRINTING-LAB REEPORT 1 23 4 56 Db L Procedure #1: a.) Look at the photo and sketch of the finished and stained paternity gel - Moodle. On the drawing to the right, sketch the location of all of the DNA bands. A2 23130 bp 9416 bp 6557 bp b.) Also on the drawing to the right, above...
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