Question

QUESTION 3.a. Could any of the reading frames in the following DNA sequence code for a protein? [This is only a portion of the full DNA sequence.] 5'-GTACCTAGGCGCGTTATATATGCGATATAGCTAGATGCTCGTAGCTGATTATATAGCTGATCGCTTGTGATGGACGTGTTA-3' 3'-CATGGATCCGCGCAATATATACGCTATATCGATCTACGACGATCGACTAATATATCGACTAGCGAACACTACCTGCACAAT-5' To help you evaluate this question, the SAME SEQUENCE is presented below with each possible reading frame indicated, remembering that there are 3 reading frames in each strand. 1..1..1..1..1..1..1..1..1..1..1..1..1..1..1..1..1..1..1..1..1..1..1..1..1..1..1.. 5'-GTACCTAGGCGCGTTATATATGCGATATAGCTAGATGCTCGTAGCTGATTATATAGCTGATCGCTTGTGATGGACGTGTTA-3' 3'-CATGGATCCGCGCAATATATACGCTATATCGATCTACGACGATCGACTAATATATCGACTAGCGAACACTACCTGCACAAT-5' 321321321321321321321321321321321321321321321321321321321321321321321321321321321 .2..2..2..2..2..2..2..2..2..2..2..2..2..2..2..2..2..2..2..2..2..2..2..2..2..2..2. 5'-GTACCTAGGCGCGTTATATATGCGATATAGCTAGATGCTCGTAGCTGATTATATAGCTGATCGCTTGTGATGGACGTGTTA-3' 3'-CATGGATCCGCGCAATATATACGCTATATCGATCTACGACGATCGACTAATATATCGACTAGCGAACACTACCTGCACAAT-5' ..3..3..3..3..3..3..3..3..3..3..3..3..3..3..3..3..3..3..3..3..3..3..3..3..3..3..3 5'-GTACCTAGGCGCGTTATATATGCGATATAGCTAGATGCTCGTAGCTGATTATATAGCTGATCGCTTGTGATGGACGTGTTA-3' 3'-CATGGATCCGCGCAATATATACGCTATATCGATCTACGACGATCGACTAATATATCGACTAGCGAACACTACCTGCACAAT-5' 5'-GTACCTAGGCGCGTTATATATGCGATATAGCTAGATGCTCGTAGCTGATTATATAGCTGATCGCTTGTGATGGACGTGTTA-3' 3'-CATGGATCCGCGCAATATATACGCTATATCGATCTACGACGATCGACTAATATATCGACTAGCGAACACTACCTGCACAAT-5' ..1..1..1..1..1..1..1..1..1..1..1..1..1..1..1..1..1..1..1..1..1..1..1..1..1..1..1 5'-GTACCTAGGCGCGTTATATATGCGATATAGCTAGATGCTCGTAGCTGATTATATAGCTGATCGCTTGTGATGGACGTGTTA-3' 3'-CATGGATCCGCGCAATATATACGCTATATCGATCTACGACGATCGACTAATATATCGACTAGCGAACACTACCTGCACAAT-5' .2..2..2..2..2..2..2..2..2..2..2..2..2..2..2..2..2..2..2..2..2..2..2..2..2..2..2. 5'-GTACCTAGGCGCGTTATATATGCGATATAGCTAGATGCTCGTAGCTGATTATATAGCTGATCGCTTGTGATGGACGTGTTA-3' 3'-CATGGATCCGCGCAATATATACGCTATATCGATCTACGACGATCGACTAATATATCGACTAGCGAACACTACCTGCACAAT-5' 3..3..3..3..3..3..3..3..3..3..3..3..3..3..3..3..3..3..3..3..3..3..3..3..3..3..3.. b. What is the name given to a reading frame that can be translated into a protein? c. Is the upper or lower strand the "transcribed" strand? d. What is the name of the other strand?

Answer 1

Answer:

A)

1st position	2nd position	3rd position
U	C	A	G
U	UUU	Phe	UCU	Ser	UAU	Tyr	UGU	Cys	U
UUC	Phe	UCC	Ser	UAC	Tyr	UGC	Cys	C
UUA	Leu	UCA	Ser	UAA	Stop	UGA	Stop	A
UUG	Leu	UCG	Ser	UAG	Stop	UGG	Trp	G
C	CUU	Leu	CCU	Pro	CAU	His	CGU	Arg	U
CUC	Leu	CCC	Pro	CAC	His	CGC	Arg	C
CUA	Leu	CCA	Pro	CAA	Gln	CGA	Arg	A
CUG	Leu	CCG	Pro	CAG	Gln	CGG	Arg	G
A	AUU	Ile	ACU	Thr	AAU	Asn	AGU	Ser	U
AUC	Ile	ACC	Thr	AAC	Asn	AGC	Ser	C
AUA	Ile	ACA	Thr	AAA	Lys	AGA	Arg	A
AUG	Met	ACG	Thr	AAG	Lys	AGG	Arg	G
G	GUU	Val	GCU	Ala	GAU	Asp	GGU	Gly	U
GUC	Val	GCC	Ala	GAC	Asp	GGC	Gly	C
GUA	Val	GCA	Ala	GAA	Glu	GGA	Gly	A
GUG	Val	GCG	Ala	GAG	Glu	GGG	Gly	G

Ala	A	Alanine
Arg	R	Arginine
Asn	N	Asparagine
Asp	D	Aspartic acid
Cys	C	Cysteine
Gln	Q	Glutamine
Glu	E	Glutamic acid
Gly	G	Glycine
His	H	Histidine
Ile	I	Isoleucine
Leu	L	Leucine
Lys	K	Lysine
Met	M	Methionine
Phe	F	Phenylalanine
Pro	P	Proline
Ser	S	Serine
Thr	T	Threonine
Trp	W	Tryptophan
Tyr	Y	Tyrosine
Val	V	Valine
Asx	B	Asn or Asp
Glx	Z	Gln or Glu
Xle	J	Leu or Ile
Sec	U	Selenocysteine (UGA)
Pyl	O	Pyrrolysine (UAG)
Unk	X	Unknown

By referring the chart given above you can easily predict the reading frames that could code for a protein

In the strand given many reading frames can codes for the amino acids like threonine,alanine,tryptophan,arginine etc…..

So these amino acids are formed together as a protein

B) Open reading frame

An open reading frame (ORF) is a reading frame that has the potential to be transcribed into RNA and translated into protein.

C )Lowest strand

The noncoding strand (also called the antisense strand, anticoding strand, template strand or transcribed strand)of DNA (3' → 5')

D) Coding strand or sense strand (5’ to 3’)

The coding strand is the DNA strand whose base sequence corresponds to the base sequence of the RNA transcript produced .It is this strand which contains codons.

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