What type of restriction enzymes should be used for methods such as DNA fingerprinting? a. all...
Homework Answers
D. Type II
Restriction endonuclease enzyme is used in finger printing , recombinant DNA technology and other biotechnological applications.
Type 1 endonuclease enzyme cut the DNA little bit away from recognition site ( about 1000 bp away). So this can't be used for specific sequence, quite needed in case of fingerprinting.
Type 3rd endonuclease enzyme is very similar to type I and cuts the DNA about 25 bp away from site..
Type 2 cut the DNA within the sequence of recognition site.
Add Answer to:
What type of restriction enzymes should be used for methods such as
DNA fingerprinting?
a. all...
Which of the following makes restriction enzymes useful for DNA fingerprinting and RFLP analysis? a. they...
Which of the following makes restriction enzymes useful for DNA fingerprinting and RFLP analysis? a. they typically attach and ligate DNA fragments together b. they cause DNA to be translated into the plasmid c. they cause DNA to be transcribed into the plasmid d. they can only function when within the cytoplasm of a cell e. none of the others are true
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answer these questions regarding PCR and DNA "fingerprinting" 1. Explain the concept of DNA” fingerprinting”. What is the reason that it is referred to as fingerprinting? 2. Explain the concept of restriction enzymes and how they are used for DNA mapping. Discuss the concept of PCR as it applies to this process. 3. Explain what is occurring at each location along the thin wires at either end in the electrophoresis box. note that fine bubbles were occurring
t Restriction enzymes can be useful for the following Biotech processes (check ALL that apply): DNA...
t
Restriction enzymes can be useful for the following Biotech processes (check ALL that apply): DNA Replication DNA Fingerprinting Genetic Engineering Insulin production using E.coli Polymerase chain reaction
SHORT ANSWER 1. Explain how the technique of DNA fingerprinting works. 2. What is the purpose...
SHORT ANSWER 1. Explain how the technique of DNA fingerprinting works. 2. What is the purpose of using a restriction enzymes? 3. What is the technique of gel electrophoresis used for? 4. What is the overall charge on DNA and what gives DNA this charge? 5. If both the parents are carriers of sickle cell disease, what is the probability of having a child that has sickle cell disease? Draw a punnett square to determine the probabilty.
What are restriction enzymes and how do they affect DNA? Why do some fragments move quickly...
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Why do restriction enzymes need to be kept on ice? What order should the DNA, enzyme,...
Why do restriction enzymes need to be kept on ice? What order should the DNA, enzyme, water and buffer be added to the microcentrifuge tube for a restriction digest? If lambda DNA is linear, how many times would the enzyme have to cut the DNA to generate five DNA fragments? Would a shorter DNA fragment move faster or slower through the agarose gel than a longer fragment? Why?
24. What do restriction enzymes do? A Randomly cut DNA B. Produce protein C. Cut DNA...
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1. A small piece of DNA was digested using restriction enzymes HindIII and PstI: A) How...
1. A small piece of DNA was digested using restriction enzymes
HindIII and PstI:
A) How many basepairs long is the fragment?
a. 900 bp
b. 2000 bp
c. 600 bp
d. 500 bp
B) How many PstI restriction sites are located within
this fragment?
a. 0
b. 3
c. 1
d. 4
e. 2
Psti Pstl Hindili 500 500 100 900
Lab 8-9 Restriction Enzymes, Gel Electrophoresis, PCR 1. Introduction: Define the following concepts and processes (use...
Lab 8-9 Restriction Enzymes, Gel Electrophoresis, PCR 1. Introduction: Define the following concepts and processes (use lecture textbook, lab manual and ppt as a resource of answers) 1) Define restriction enzyme and how they work 2) Define RFLP. What is full name of RFLP? What is its application? 3) Define DNA fingerprinting and its application 4) Define PCR. What is full name of PCR? What is its application? 5) Define STR and its application 6) Compare and contrast the two...
14. Restriction endonucleases are a. enzymes that restrict DNA synthesis b. enzymes that cut DNA in...
14. Restriction endonucleases are a. enzymes that restrict DNA synthesis b. enzymes that cut DNA in specific sequences c. nuclear proteins that are involved in transcription d. components of the ribosomes involved in protein synthesis 15. The first step in southern blotting is a. converting DNA into RNA b. cutting high molecular weight DNA into smaller pieces c. converting RNA into DNA d. radioactively labeling the DNA so it can be detected after the procedure is complete 16. The major...
t
Restriction enzymes can be useful for the following Biotech processes (check ALL that apply): DNA Replication DNA Fingerprinting Genetic Engineering Insulin production using E.coli Polymerase chain reaction
SHORT ANSWER 1. Explain how the technique of DNA fingerprinting works. 2. What is the purpose of using a restriction enzymes? 3. What is the technique of gel electrophoresis used for? 4. What is the overall charge on DNA and what gives DNA this charge? 5. If both the parents are carriers of sickle cell disease, what is the probability of having a child that has sickle cell disease? Draw a punnett square to determine the probabilty.
24. What do restriction enzymes do? A Randomly cut DNA B. Produce protein C. Cut DNA at a specific nucleotide sequence D. Copy DNA 25. Which of the following is an example of a differentiated cell? A. Embryonic Stem Cell B. Hematopoeitic Stem Cell C. Adult Stem Cell D. Liver Cell 26. An example of a small, circular DNA molecule used as a vector to transfer foreign DNA to a host cell is a A. plasmid B prion C. liposome...
1. A small piece of DNA was digested using restriction enzymes
HindIII and PstI:
A) How many basepairs long is the fragment?
a. 900 bp
b. 2000 bp
c. 600 bp
d. 500 bp
B) How many PstI restriction sites are located within
this fragment?
a. 0
b. 3
c. 1
d. 4
e. 2
Psti Pstl Hindili 500 500 100 900
Lab 8-9 Restriction Enzymes, Gel Electrophoresis, PCR 1. Introduction: Define the following concepts and processes (use lecture textbook, lab manual and ppt as a resource of answers) 1) Define restriction enzyme and how they work 2) Define RFLP. What is full name of RFLP? What is its application? 3) Define DNA fingerprinting and its application 4) Define PCR. What is full name of PCR? What is its application? 5) Define STR and its application 6) Compare and contrast the two...
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