SNP - This stands for 'single nucleotide polymorphisms '
It is a single nucleotide variation in the DNA of the genome of the individual. Each of these variations are more than 1% in the population.
RFLP - Restrictions fragment length polymorphisms- it is a technique, particularly used in molecular biology, to study the variations in the homologous DNA sequences.
This method hepls to find minor differences between individuals ,populations and species.
These sequences also help to find particular gene sequences in the genome.
HOW CAN THESE BE USED TO FIND THE PARTICULAR GENE OF INTEREST.
These act by trail and error method. And this also requires particular probes and enzymes.
describe a SNP and a RFLP. How can these be used to locate a gene of...
How can Southern Blotting, a type of RFLP, be used when dealing with plasmid DNA? Do you use the plasmid as a molecular probe?
Anatomically describe how to locate the rhomboid muscles on the cat. Anatomically describe how to locate the brachioradialis muscle on the cat. Anatomically describe how to locate the four hamstring muscles on the cat. Anatomically describe how to locate and determine the four abdominal muscles on the cat.
How is it possible that different mutations can result in the loss of a RFLP? How are positive YAC clones selected? What is the purpose of digesting the YAC with BamH and SnaB1?
Describe three mechanisms of regulation of gene expression in eukaryotes that are not used by prokaryotes. Give examples.
Questions? BIOTECHNOLOGY: Describe and explain what restriction enzymes are? what their important characteristic are, and how they work, and how they can be used for forensic applications( RFLP analysis) Explain and understand gel electrophoresis, how does IT sort Dna by size? You should understand what PCR is and the basic of how IT works and its forensic applications (STR analysis) of present with a gel fragment pattern, you should be able to interpret it.
. If an adenoviral vector is used for gene therapy, why might it be necessary to give the patient multiple rounds? 4. How can a “suicide gene” be used to treat cancer? 5. Rexin-G is an effective vector for tumor treatment. Describe its 2 primary modes of action.
Describe how homologous recombination can change DNA sequences by gene conversion vs. how it can cause deletions/insertions.
8. Explain how SNP genotyping can improve patient outcomes when prescribing drugs.
Describe and outline the structural components of a gene, include all regulatory regions that can impact transcription initiation. Then add the chromatin structure, making sure to describe the structure and role of the nucleosome. Now, describe the process of how a gene is actively expressed starting with transcription initiation and ending with a protein product (be specific about each step). Lastly, describe three different mechanisms of regulation of this process and the possible outcomes of each.
9. Describe the role of transcription factors in gene expression and explain how transcription factors can allow cells to be different from each other.