What enzyme activities and DNAs are required to clone a fragment of mouse DNA?

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What enzyme activities and DNAs are required to clone a fragment of mouse DNA?

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Answer 1

Answer #1

The things that would be required for mouse DNA cloning

1. Plasmid DNA where the mouse DNA needs to be cloned

2. Primer DNA oligos which helps in amplifying the region of interest from mouse DNA

3. Suitable restriction endonucleases that makes uniques cuts in the plasmid and at two ends of the amplifies DNA.

4. TaqDNA polymerase for PCR amplification of target DNA

5. DNA ligase for ligating the traget DNA into plasmid DNA.

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Answer 2

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What enzyme activities and DNAs are required to clone a fragment of mouse DNA?

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