Q4) A) Write the complement (also known as inverse complement) of the sequence below indicating its...
Q4)
A) Write the complement (also known as inverse complement) of
the sequence below
indicating its
directionality-5’-ACGGGCATTAGACATCCACGGAAATTCGCAG
B)Mark the recognition site(s) in this sequence for the restriction
enzyme FokI which
recognizes the sequence GGATG
Homework Answers
Ans 4)
A) Given sequence : 5'-ACGGGCATTAGACATCCACGGAAATTCGCAG-3'
complement sequence : 3'-TGCCCGTAATCTGTAGGTGCCTTTAAGCGTC-5'
B) Recognition site of Fokl is GGATG, so , recognition sites in the complement sequence would be:
3'-TGCCCGTAATCTGTAGGTGCCTTTAAGCGTC-5'
A) The complement of a DNA sequence is formed by replacing each base with its complementary base. The complementary bases are as follows: A (adenine) pairs with T (thymine), and C (cytosine) pairs with G (guanine). Additionally, the directionality of the sequence is indicated by the 5' and 3' ends.
Given sequence: 5’- ACGGGCATTAGACATCCACGGAAATTCGCAG -3’
Complement: 3’- TGCCCGTAATCTGTAGGTGCCGTTTAAGCGT -5’
B) The recognition site for the restriction enzyme FokI is "GGATG". To find this site in the given sequence, we need to look for the occurrence of "GGATG".
The sequence contains the recognition site "GGATG" at position 18 to 22 (reading from the 5' end). The sequence "GGATG" is located between the bases C and A in the given sequence.
So, the recognition site for the restriction enzyme FokI in the given sequence is:
5'- ACGGGCATTAGACATCCAGGATGGAAATTCGCAG -3'
Add Answer to:
Q4)
A) Write the complement (also known as inverse complement) of
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The plasmid pFR55 is a useful vehicle for the cloning
of any DBA sequence in E. coli. A restriction map of pFR55 showing
the restriction enzyme cutting sites is shown below. The plasmid
can replicate I'm E. coli and carries the tetracycline resistance
Tc and ampicillin resistance (Ap) genes for use as selectable
markers in E. coli.
a. you wish to insert a gene into the SalI site of
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hi I need help with these questions please
q1 How long is the DNA sequence encoding the protein that
confers ampicillin resistance (in bp units)? Input one number only,
with no spaces or units. (1 mark)
q2 How long is the DNA sequence encoding the protein that
confers hygromycin resistance (in bp units)? Input one number only,
with no spaces or units. (1 mark)
q3 What is the size of the OTC-Δ DNA sequence that has been
inserted into the...
1. The figure below shows a DNA double helix and its nucleotide
sequence.
A. A homodimeric helix–turn–helix protein binds to a DNA
fragment containing this sequence. Its preferred half-site is
AACAC. Show where the two recognition helices in the protein
contact the DNA.
B. Are protein–DNA contacts primarily in the major or the minor
groove of DNA? What parts of the nucleotides contact the amino acid
side chains of the protein to provide most sequence specificity?
What kind of bond...
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