What ensures that the bacteriophage genes we have PCR amplified are cloned into the pSMEG vector...
What ensures that the bacteriophage genes we have PCR amplified are cloned into the pSMEG vector in the proper orientation, meaning that the translation start codon is oriented close to the promoter and not the other way around.
Homework Answers
We need to cut the clone with specific restriction enzymes that will cut on one side of the inserted fragment;i.e on the vector, near to the inserted fragment and also cut on the backbone of the insert.
Agarose gel electrophoresis to resolve the fragments can be done amd the size of the bands can be analyzed after gel run under UV. This would clearly give an idea about the orientation of the inserted gene within the pSMEG vector.
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What ensures that the bacteriophage genes we have PCR amplified
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Use the following information
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work for thumbs up.
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Use the following information
To help you solve the following questions. Show all
work for thumbs up.
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