1) The minor groove of A-DNA is shallower than its B-DNA counterpart. Would this favor the...
1) The minor groove of A-DNA is shallower than its B-DNA counterpart. Would this favor the prevalence of transcription factor interactions with the minor groove of A-DNA over interactions with its major groove?
Homework Answers
The size of the major or minor groove determines the accessibility of the DNA towards various DNA binding factors including transcription factors. In case of A-DNA, the minor groove is shallower which suggests that transcription factors will have lesser accessibility to this groove. The major groove, on the other hand, has larger size and is thus more accessible to transcription factors. Thus, the transcription factors will primarily bind to the major groove as compared to the minor groove.
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1) The minor groove of A-DNA is shallower than its B-DNA
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fragment containing this sequence. Its preferred half-site is
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What part(s) of a nucleotide will occupy the major & minor groove of a double-stranded DNA molecule? WHY? What parts are found in the DNA backbone? WHY? Where would a Restriction Endonuclease associate with DNA? How long is a double-stranded DNA molecule that is 2 x 105 bp? How many nucleosomes would be required to package this DNA if it were in a Eukaryote? When chromatin from any eukaryote is digested briefly with micrococcal nuclease (an endonuclease) and fractionated using...
1. The figure below shows a DNA double helix and its nucleotide
sequence.
A. A homodimeric helix–turn–helix protein binds to a DNA
fragment containing this sequence. Its preferred half-site is
AACAC. Show where the two recognition helices in the protein
contact the DNA.
B. Are protein–DNA contacts primarily in the major or the minor
groove of DNA? What parts of the nucleotides contact the amino acid
side chains of the protein to provide most sequence specificity?
What kind of bond...
DNA is the molecule used to store the genetic code of most living organisms on earth. Its structure was famously proposed by Watson and Crick who proposed double helix structure published in the journal Nature, April 25, 1953. For this the Nobel Prize was awarded to Watson, Crick & Wilkins in 1962. (a) (i) (ii) Show the chemical structure of the DNA backbone. What is a 1. the backbone, 2. a nucleoside, 3. a nucleotide 4. a nucleic acid? (b)...
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