Which of the following would not be expected to have an effect on the activity of...
Which of the following would not be expected to have an effect on the activity of ß-galactosidase?
| a. |
Absorbance |
|
| b. |
pH |
|
| c. |
ONPG (ortho-nitrophenol-ß-galactoside) concentration |
|
| d. |
BSA concentration |
|
| e. |
Temperature |
|
| f. |
ß-Galactosidase concentration |
Homework Answers
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Which of the following would not be expected to
have an effect on the activity of...
10) Would an amino acid with the given side chain be most likely found in the hydrophobic or hydrophilic region of a pr...
10) Would an amino acid with the given side chain be most likely found in the hydrophobic or hydrophilic region of a protein? -CH-CH3 он CH2-CH-CH3 он CH3 11) What process occurs when heat, acids, bases, and heavy metal ions cause loss of biological function of a protein? 12) Answer the following questions regarding enzyme activity: a) The optimum temperature and pH for most biological enzymes is b) The molecules to which an enzyme binds are called the c) The...
16 If other conditions were to remain constant, which one of the following changes would explain...
16 If other conditions were to remain constant, which one of the following changes would explain a reduced rate of activity in an enzyme -catalysed reaction? [-A-] an increase in the concentration of the end products [-B-] an increase in the concentration of the substrate [-C-] an increase in the concentration of the enzyme [-D-] an increase in the temperature towards the optimum. [-E-] an increase in the pH towards the optimum
Which of the following is expected to have the highest yield of ONP in the enzyme...
Which of the following is expected to have the highest yield of ONP in the enzyme kinetics experiment? 5.00 ml of PO4,0.50 ml of ONPG, 0.50 ml of lactase = 6 ml 1 2.00 ml of PO4, 4.0 ml of ONPG, 0.00 ml of lactase = 6 ml 5.50 ml of PO4, 0.00 ml of ONPG,0.50 ml of lactase - 6 ml 5.25 ml of PO4, 0.25 ml of ONPG, 0.50 ml of lactase 6 ml
Following intensive exercise, which of the following would be expected to occur? A.Increased production of 2,3-BPG...
Following intensive exercise, which of the following would be expected to occur? A.Increased production of 2,3-BPG B. Decreased hemoglobin affinity for oxygen C. Allosteric inhibition of hemoglobin via binding of H+ ions D. All of the above 2. A high concentration of H+ ions will have what kind of effect on the hemoglobin oxygen dissociation curve? a - A shift to the right A shift to the left b- No effect c- It is not possible to predict with the...
Q4. Which of the following compounds are expected to form interamolecular hydrogen bonds? Show the hydrogen...
Q4. Which of the following compounds are expected to form interamolecular hydrogen bonds? Show the hydrogen bond formed in each case? a) Ortho cresol b) Ortho nitroaniline c) salicylic acid d) salicyladehyde e) o-florophenol f) o-hydroxybenzonitrile
to form Which of the following possible Birch reduction products would not be expected CH3 CH3...
to form Which of the following possible Birch reduction products would not be expected CH3 CH3 0 CH3 A. B. C. Which of the following substrates would most likely undergo a successful nucleophilic aromatic substitution reaction with diethylamine? NO2 NO 2 NO 2 A. B. C. D. Which of the following resonance structures does not depict stabilization of the negative charge in the Meisenheimer complex? 0 A. The nitro group has which of the following effects in electrophilic aromatic substitution?...
-2. Which of the following tripeptides would carry a net charge of-1 at pH 7? Markallthatapply....
-2. Which of the following tripeptides would carry a net charge of-1 at pH 7? Markallthatapply. (3 pts.) A. Asp-Ala-Arg C. Lys-Gly-Asn D. Glu-Lys-Asp E. Leu-Asp-Glu F. Arg-lleu-Cys B. Met-Pro-Glu The Kd for a dimeric protein complex is 2.2 μM. (3 pts.) Mark all that are true about this dimeric protein complex. A. 3. At equilibrium, the concentration of the individual subunits is very, very low. B. If this is a homodimer, it can be called an αβ dimer. C....
QUESTION 1 Match each E10 reaction component with its role. A. substrate Nitrophenyl phosphate stops reaction...
QUESTION 1 Match each E10 reaction component with its role. A. substrate Nitrophenyl phosphate stops reaction and allows absorbance of product to be B. measured Nitrophenol Acid phosphatase C. makes pH optimal for enzyme activity 1.5% KOH D. enzyme E. product QUESTION 2 This experiment assays the reaction rate of wheat germ acid phosphatase at a temperature of [a]°C.
EFFECT OF TEMPERATURE: 1. Bubble height indicates the degree of enzyme activity. Based on the results...
EFFECT OF TEMPERATURE:
1. Bubble height indicates the degree of enzyme activity. Based
on the results of your experiment, which tube had the highest
bubble column height? What can you conclude about this
temperature?
2. Compare the results of the tubes which were left on ice, at
25ºC, and at 37ºC. What can you conclude about the effect of
temperature on enzyme activity?
3. What happened in the tube with boiled catalase? Explain this
result.
Results: Table 5.1 - Catalase...
Predict the effect the substituent attached to the benzene ring below would have on electrophilic aromatic...
Predict the effect the substituent attached to the benzene ring below would have on electrophilic aromatic substitution reactions? CH, CH3 a) meta director, deactivator b) ortho/para director, activator c) meta director, activator d) ortho/para director, deactivator
10) Would an amino acid with the given side chain be most likely found in the hydrophobic or hydrophilic region of a protein? -CH-CH3 он CH2-CH-CH3 он CH3 11) What process occurs when heat, acids, bases, and heavy metal ions cause loss of biological function of a protein? 12) Answer the following questions regarding enzyme activity: a) The optimum temperature and pH for most biological enzymes is b) The molecules to which an enzyme binds are called the c) The...
16 If other conditions were to remain constant, which one of the following changes would explain a reduced rate of activity in an enzyme -catalysed reaction? [-A-] an increase in the concentration of the end products [-B-] an increase in the concentration of the substrate [-C-] an increase in the concentration of the enzyme [-D-] an increase in the temperature towards the optimum. [-E-] an increase in the pH towards the optimum
Which of the following is expected to have the highest yield of ONP in the enzyme kinetics experiment? 5.00 ml of PO4,0.50 ml of ONPG, 0.50 ml of lactase = 6 ml 1 2.00 ml of PO4, 4.0 ml of ONPG, 0.00 ml of lactase = 6 ml 5.50 ml of PO4, 0.00 ml of ONPG,0.50 ml of lactase - 6 ml 5.25 ml of PO4, 0.25 ml of ONPG, 0.50 ml of lactase 6 ml
to form Which of the following possible Birch reduction products would not be expected CH3 CH3 0 CH3 A. B. C. Which of the following substrates would most likely undergo a successful nucleophilic aromatic substitution reaction with diethylamine? NO2 NO 2 NO 2 A. B. C. D. Which of the following resonance structures does not depict stabilization of the negative charge in the Meisenheimer complex? 0 A. The nitro group has which of the following effects in electrophilic aromatic substitution?...
-2. Which of the following tripeptides would carry a net charge of-1 at pH 7? Markallthatapply. (3 pts.) A. Asp-Ala-Arg C. Lys-Gly-Asn D. Glu-Lys-Asp E. Leu-Asp-Glu F. Arg-lleu-Cys B. Met-Pro-Glu The Kd for a dimeric protein complex is 2.2 μM. (3 pts.) Mark all that are true about this dimeric protein complex. A. 3. At equilibrium, the concentration of the individual subunits is very, very low. B. If this is a homodimer, it can be called an αβ dimer. C....
QUESTION 1 Match each E10 reaction component with its role. A. substrate Nitrophenyl phosphate stops reaction and allows absorbance of product to be B. measured Nitrophenol Acid phosphatase C. makes pH optimal for enzyme activity 1.5% KOH D. enzyme E. product QUESTION 2 This experiment assays the reaction rate of wheat germ acid phosphatase at a temperature of [a]°C.
EFFECT OF TEMPERATURE:
1. Bubble height indicates the degree of enzyme activity. Based
on the results of your experiment, which tube had the highest
bubble column height? What can you conclude about this
temperature?
2. Compare the results of the tubes which were left on ice, at
25ºC, and at 37ºC. What can you conclude about the effect of
temperature on enzyme activity?
3. What happened in the tube with boiled catalase? Explain this
result.
Results: Table 5.1 - Catalase...
Predict the effect the substituent attached to the benzene ring below would have on electrophilic aromatic substitution reactions? CH, CH3 a) meta director, deactivator b) ortho/para director, activator c) meta director, activator d) ortho/para director, deactivator
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