what is a more sensitive process ? coomassie blue staining or immunoblotting of Hsp70 proteins? is...
Homework Answers
Coomassie blue staining is used to visualise the protein after SDS PAGE to determine its molecular weight and its relative amount. Its can detect protein 0.3ug/band. It stains all types of proteins. While in case of immunoblotting, protein from gel after SDS PAGE are transferred to PVDF membrane and then detected using specific primary antibody against Hsp70 only followed by secondary antibody conjugated with enzyme that gives colored product to visualise the protein band. It can acheive sensitivies of 1ng/band.
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what is a more sensitive process ? coomassie blue staining or
immunoblotting of Hsp70 proteins? is...
Exercise IV. Fill in the Blank 1. The method of Centrifugation, polyacrylamide gel electrophoresis, western blotting,...
Exercise IV. Fill in the Blank 1. The method of Centrifugation, polyacrylamide gel electrophoresis, western blotting, affinity purification) is the most widely used technique for determining the approximate molecular weight of a protein. 2. (Centrifugation, affinity chromatography, sonication, gel electrophoresis) is a method in which macromolecules are separated due to their size, charge, and other physical properties 3. SDS-PAGE is a form of electrophoresis in the presences of a/an (acidic solution, basic solution, anionic detergent, cationic detergent). 4. SDS not...
You use the Bradford (coomassie) assay to determine the concentration of Protein A. You use bovine...
You use the Bradford (coomassie) assay to determine the concentration of Protein A. You use bovine albumin as your standard. You determine the Protein A concentration to be 2 mg/ml. When you use Protein A as your standard, you determine the Protein A concentation of your unknown to be 3 mg/ml. Explain the discrepancy (pick ONE answer): (2 points) a. Protein A has fewer basic amino acids than albumin. b. Protein A has more acidic amino acids than albumin c....
Assume that Citizens’ assets are more rate sensitive than its liabilities. What will the impact be...
Assume that Citizens’ assets are more rate sensitive than its liabilities. What will the impact be on Net Interest Margin if interest rates increase? EXPLAIN
Please describe the technique thoroughly and give examples of proteins that would be involved in ...
Please describe the technique thoroughly and give examples of proteins that would be involved in the analysis. Limitations/advantages? how do you differentiate or if its good for small or large amino acids? Protein Analysis: For each of the techniques, we covered you should understand the basics of what the technique can be used to discover SDS-Page/ coomassie blue Western blot (focus on the part after SDS-PAGE) Protease Digestion & Mass Spec Crystallography Edman Degradation NMR I may ask you to...
What is the decolorizer used in acid-fast cell staining? Carbolic Acid (PhOH) and Ethanol (EtOH) Acetic...
What is the decolorizer used in acid-fast cell staining? Carbolic Acid (PhOH) and Ethanol (EtOH) Acetic Acid (AcOH) and Ethanol (EtOH) Hydrochloric Acid (HCl) and Acetone (Ace) Carbolic Acid (PhOH) and Acetone (Ace) Hydrochloric Acid (HCl) and Ethanol (EtOH) Acetic Acid (AcOH) and Acetone (Ace) Endospores are formed by ____ cells. mother cells host cells parental cells filial cells maternal Upon competition of the endospore staining procedure, the endospore will appear A. pink. B. purple. C. green. D. unstained. Which...
Explain why ICP-MS is more sensitive than ICP-AES. Please explain why, do not just compare them,...
Explain why ICP-MS is more sensitive than ICP-AES. Please explain why, do not just compare them, thanks!
Other than hydrogen bonding, what is the major interaction that causes proteins to fold into compact,...
Other than hydrogen bonding, what is the major interaction that causes proteins to fold into compact, well-ordered structures? In no more than 5 sentences, explain how this interaction results in the folding of proteins.
Describe why AES is more sensitive to temperature changes than AAS. (HINT: Think about what AES...
Describe why AES is more sensitive to temperature changes than AAS. (HINT: Think about what AES measures compared to AAS.) PLEASE DESCRIBE IN DETAIL
What properties of enzymes make them more suitable than structural proteins for experimental studies of the...
What properties of enzymes make them more suitable than structural proteins for experimental studies of the regulation of expression of their genes? There may be more than one correct answer - indicate ALL the answers you think are CORRECT. A- Enzymes produce products, structural proteins do not. B- Amounts of enzymes change in response to concentrations of metabolites. C- Enzymes are more abundant in the cell than structural proteins. D- Enzymes cannot be detected using antibody methods. E- Products of...
LABORATORY REPORT EXERCISE 9 Name NEGATIVE STAINING Make a drawing below of one field of bacteria...
LABORATORY REPORT EXERCISE 9 Name NEGATIVE STAINING Make a drawing below of one field of bacteria from each side. Use the proper color for the background Congo red Stain Nigrosin Stain 2. WIIIIIII Other than bacteria, what other microorganisms might one encounter in the mouth? 3. Why does the negative stain give a more accurate estimation of bacterial size than other stains? Name a pathogenic spirochete and the disease it causes. Genus Species Disease Why is it difficult to cultivate...
Exercise IV. Fill in the Blank 1. The method of Centrifugation, polyacrylamide gel electrophoresis, western blotting, affinity purification) is the most widely used technique for determining the approximate molecular weight of a protein. 2. (Centrifugation, affinity chromatography, sonication, gel electrophoresis) is a method in which macromolecules are separated due to their size, charge, and other physical properties 3. SDS-PAGE is a form of electrophoresis in the presences of a/an (acidic solution, basic solution, anionic detergent, cationic detergent). 4. SDS not...
LABORATORY REPORT EXERCISE 9 Name NEGATIVE STAINING Make a drawing below of one field of bacteria from each side. Use the proper color for the background Congo red Stain Nigrosin Stain 2. WIIIIIII Other than bacteria, what other microorganisms might one encounter in the mouth? 3. Why does the negative stain give a more accurate estimation of bacterial size than other stains? Name a pathogenic spirochete and the disease it causes. Genus Species Disease Why is it difficult to cultivate...
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