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A linear piece of DNA was broken into random, overlapping fraqments and each fragment was then sequenced. The sequence of each fragment is shown below, listed in a random order 5-ATCAAAAGC-3 5-TC 5-GAGGTACCCTTC-3 5-CTATCAAAAGCAT-3 5-AAAAGCATAGAGGTACC-3 5-AAGCATAGAGGTACC-3 TAGAGGTACC-3 af the original fragment placing the sequence that is closest to the 5 end first and moving towards the 3 end. 5 end 5-ATCAAAAGC-3 5-AAAAGCATAGAGGTACC-3 5-TCAAAAGCATAGAGGTACC-3 5-CTATCAAAAGCAT-3 5-GAGGTACCCTTC- 5-AAGCATAGAGGTACC-3 3 and Incorrect You have incorrectly placed the sequence CAAAGCAT. This sequence is the most 5 fragment and should be placed first.
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Answer #1

The coding sequence of the original fragment can be determined by observing the overlapping regions and adding the sequences to the 5' end and 3' end.
Realign the sequences in the following order,
5'CTATCAAAA3'
5'ATCAAAAGC3'
5'TCAAAAGCATAGAGGTACC3'
5'AAAAGCATAGGAGGTACC3'
5'AAGCATAGGAGGTAC3'
5'GAGGTACCCTTC3'
hence the coding sequence is
5'CTATCAAAAGCATAGAGGTACCCTTC3'

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