(Choose the best answer) A polylinker increases the versatility of a DNA plasmid because it contains:...
| (Choose the best answer) A polylinker increases the versatility of a DNA plasmid because it contains: | |||||||||||
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| cDNA libraries | |||||||||||
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Homework Answers
1. Option a is correct. Polylinker contains a DNA recognition sequence for several different restriction enzymes.
2. Option d is correct. cDNA libraries require reverse transcriptase for their construction. cDNA libraries are prepared from the mRNA using the reverse transcriptase.
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(Choose the best answer) A polylinker increases the versatility
of a DNA plasmid because it contains:...
A polylinker increases the versatility of a DNA plasmid because it: (select all correct answers) A)...
A polylinker increases the versatility of a DNA plasmid because it: (select all correct answers) A) facilitates the cloning of DNA segments. B) contains a DNA sequence that confers resistance to ampicillin. C) contains a DNA sequence to allow the vector to replicate. D)contains DNA recognition sequences for several different restriction enzymes. 2. You have an E. coli plasmid whose sequence contains three EcoRI restriction sites. How many DNA fragments would result from a complete EcoRI restriction enzyme digest? a....
A plasmid used as a cloning vector in E. coli must have… Does sequence similarity between...
A plasmid used as a cloning vector in E. coli must have… Does sequence similarity between genes play an important role in assigning gene function? Successful insertion of a DNA fragment into the multi-cloning region (restriction sites) of a recombinant plasmid is detected by what changes? Understand the concept of (restriction enzyme produced) DNA fragment separation by gel electrophoresis. In addition to restriction enzymes, which enzyme(s) are required to insert a fragment of DNA into a cloning vector? What is...
I didnt do quite as well as i wpuld have liked on my molecular bio exam....
I
didnt do quite as well as i wpuld have liked on my molecular bio
exam. Please help me reciew what I missed. Thanks!
We were unable to transcribe this imageWe were unable to transcribe this imageA fragment restricted with EcoRI enzyme can be used for ligation into a plasmid that was restricted with BamHI because both the insert and the plasmid contain sticky ends a True b) False 10. Polynucleotide probes can be used to screen a genomic library...
please answer all amils A Doon E Conjagants DNA hips o sloning of freign DA fa...
please answer all
amils A Doon E Conjagants DNA hips o sloning of freign DA fa ts are called C Clne D Vecto 20 Whatkind of termini is produced by the retriction endonelease Prl, which restriction site has is 5CGATIC (1he dowswd aow represents the site of cleavage in each strand) D Reverse ends E Polylinker termini A.Y ovehan Byaverhang C Blant ends 21.Eeryme dhat cleave DNA at seuence-specifie sites is called D Exonuclease E Integrase A DNA polymerase Ligase...
command 145 Genetics Assignment Genomic Analysis 1. Some bacteria normally produce endonucleases for what purpose? a)...
command 145 Genetics Assignment Genomic Analysis 1. Some bacteria normally produce endonucleases for what purpose? a) necessary digestion of their own genome b) defense against viral infection c) bacteria never make endonucleases naturally 2. A blunt cutter produces DNA fragments with complementary overhanging regions. a) True b) False 3. Which of the following DNA sequences (when double-stranded) most likely represents a restriction endonuclease site for a 6-mer cutter? a) AGTAAGCTTC c) AGAGAGCCAA b) GGTAGATTCC d) None of the above 4....
KpnI (255) lacz alpha gene ECORI (265) Psti (270) PUC origin Smal (3660) рвозобw 3973 bp...
KpnI (255) lacz alpha gene ECORI (265) Psti (270) PUC origin Smal (3660) рвозобw 3973 bp EcoRI (875) Amp(R) Psti (1550) Kan(R) Ncol (1275) 8. You will be using the plasmid shown above as a vector for a DNA cloning experiment. You will create a library of genomic DNA fragments that will then be ligated into this plasmid. The plasmid carries two antibiotic resistance genes, Amp(R) and Kan(R), an origin of replication and a LacZ gene (Lac Z alpha). The...
Chromosomal and plasmid DNA can be cut into manageable pieces by restriction enzymes. Using agarose gel...
Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
cDNA libraries are constructed by using reverse transcriptase to synthesize DNA from mRNA that has been...
cDNA libraries are constructed by using reverse transcriptase to synthesize DNA from mRNA that has been isolated from a cell. Since cDNA libraries represent mRNA sequences found within a cell, which of the following would be missing from a gene's sequence in a eukaryotic cDNA library? A) a start codon B) exons C) a signal sequence for polyadenylation D) introns E) a stop codon The following results were achieved after performing an Ames test by exposing his- bacteria to four...
Identify two restriction endonucleases that could be used to make sticky ends near the 5’ end of this DNA sequence (uppe...
Identify two restriction
endonucleases that could be used to make sticky ends near the
5’ end of this DNA sequence (upper strand) so that
it could be incorporated into a new plasmid. You have a short list
of them in Table 9-2, and the specific, short sequences of bases
that other enzymes cut at are easily obtained from web resources.
You must cut as near to the 5' end as possible. Indicate the
specific sequences of bases for each endonuclease...
24. What would be the anticodon if the template strand of DNA Is ACC A UCC...
24. What would be the anticodon if the template strand of DNA Is ACC A UCC B.) TGG UGG D. ACC E. TCC 25. Prior to protein synthesis, the DNA A. attracts tRNAs with appropriate amino acids. 6.) serves as a template for the production of mRNA. C. adheres to ribosomes for protein synthesis. D. contains anticodons that become codons. E. must first undergo replication. 26. The Human Genome Project has revealed that human DNA has approximately A. 30,000 bases...
I
didnt do quite as well as i wpuld have liked on my molecular bio
exam. Please help me reciew what I missed. Thanks!
We were unable to transcribe this imageWe were unable to transcribe this imageA fragment restricted with EcoRI enzyme can be used for ligation into a plasmid that was restricted with BamHI because both the insert and the plasmid contain sticky ends a True b) False 10. Polynucleotide probes can be used to screen a genomic library...
please answer all
amils A Doon E Conjagants DNA hips o sloning of freign DA fa ts are called C Clne D Vecto 20 Whatkind of termini is produced by the retriction endonelease Prl, which restriction site has is 5CGATIC (1he dowswd aow represents the site of cleavage in each strand) D Reverse ends E Polylinker termini A.Y ovehan Byaverhang C Blant ends 21.Eeryme dhat cleave DNA at seuence-specifie sites is called D Exonuclease E Integrase A DNA polymerase Ligase...
command 145 Genetics Assignment Genomic Analysis 1. Some bacteria normally produce endonucleases for what purpose? a) necessary digestion of their own genome b) defense against viral infection c) bacteria never make endonucleases naturally 2. A blunt cutter produces DNA fragments with complementary overhanging regions. a) True b) False 3. Which of the following DNA sequences (when double-stranded) most likely represents a restriction endonuclease site for a 6-mer cutter? a) AGTAAGCTTC c) AGAGAGCCAA b) GGTAGATTCC d) None of the above 4....
KpnI (255) lacz alpha gene ECORI (265) Psti (270) PUC origin Smal (3660) рвозобw 3973 bp EcoRI (875) Amp(R) Psti (1550) Kan(R) Ncol (1275) 8. You will be using the plasmid shown above as a vector for a DNA cloning experiment. You will create a library of genomic DNA fragments that will then be ligated into this plasmid. The plasmid carries two antibiotic resistance genes, Amp(R) and Kan(R), an origin of replication and a LacZ gene (Lac Z alpha). The...
Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
cDNA libraries are constructed by using reverse transcriptase to synthesize DNA from mRNA that has been isolated from a cell. Since cDNA libraries represent mRNA sequences found within a cell, which of the following would be missing from a gene's sequence in a eukaryotic cDNA library? A) a start codon B) exons C) a signal sequence for polyadenylation D) introns E) a stop codon The following results were achieved after performing an Ames test by exposing his- bacteria to four...
Identify two restriction
endonucleases that could be used to make sticky ends near the
5’ end of this DNA sequence (upper strand) so that
it could be incorporated into a new plasmid. You have a short list
of them in Table 9-2, and the specific, short sequences of bases
that other enzymes cut at are easily obtained from web resources.
You must cut as near to the 5' end as possible. Indicate the
specific sequences of bases for each endonuclease...
24. What would be the anticodon if the template strand of DNA Is ACC A UCC B.) TGG UGG D. ACC E. TCC 25. Prior to protein synthesis, the DNA A. attracts tRNAs with appropriate amino acids. 6.) serves as a template for the production of mRNA. C. adheres to ribosomes for protein synthesis. D. contains anticodons that become codons. E. must first undergo replication. 26. The Human Genome Project has revealed that human DNA has approximately A. 30,000 bases...
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