Suppose the restriction enzyme GsuII binds a site that is 2 nucleotides in length. How often...
Suppose the restriction enzyme GsuII binds a site that is 2 nucleotides in length. How often (how many base pairs) does this enzyme cut DNA?
Your answer must be numeric.
Homework Answers
Number of different types of bases in DNA = 4
The formula for calculating the probability for finding a
restriction site = 1/4n
n = number of bases in the recognition sequence
If a restriction enzyme recognizes a dinucleotide sequence, n =
2
= 1/42
= 1/16
There is a theoretical probability of finding a recognition site
for every 16 nucleotides.
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Suppose the restriction enzyme GsuII binds a site that is 2
nucleotides in length. How often...
Suppose the restriction enzyme Gsull binds a site that is 3 nucleotides in length. How often...
Suppose the restriction enzyme Gsull binds a site that is 3 nucleotides in length. How often (how many base pairs) does this enzyme cut DNA? Your answer must be numeric.
1. A circular plasmid has two PmeI restriction sites. A PmeI restriction enzyme will cut this...
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1. If a restriction enzyme cuts a circular plasmid twice, how many fragments would you see...
1. If a restriction enzyme cuts a circular plasmid twice, how many fragments would you see on the gel? 2. How would you estimate the total number of base pairs in a plasmid by looking at the DNA fragments of the digested plasmid on a gel? 3. If a linear 1kb DNA fragment has a restriction site that is located 50 bp from one end of the plasmid, what would you expect to see if the digested and undigested DNA...
EcoRI is a common restriction enzyme used in cloning experiments. Its restriction sequence is G’AATTC. The...
EcoRI is a common restriction enzyme used in cloning experiments. Its restriction sequence is G’AATTC. The strand is cut at the position of the apostrophe. The 50 base pair sequence shown below contains one or more EcoRI sites. Find them and circle them. Then count the resulting size (in base pairs – bp) of the DNA fragments (pieces) left over after using EcoRI to cut this DNA sequence. Circle the EcoRI sites in the sequence below. 1- ggagaattcgctgtacgaggttaaccccgatgccATGGCATGAATTCGTG -50 List...
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Suppose you are going to do a restriction digest with a plasmid, using the restriction enzyme...
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A linear DNA molecule that is 3250 bp long is digested with restriction enzyme A alone,...
A linear DNA molecule that is 3250 bp long is digested with restriction enzyme A alone, restriction enzyme B alone, or a combination of enzymes A and B to produce the following fragment sizes. Construct a restriction map of the DNA fragment. How many times does enzyme A cut the linear DNA molecule? If you are unable to place both enzymes A and B on the map, show one possible arrangement of enzyme A cognition sites on the DNA molecule.
Help with these two would be appreciated JUVIJVVEL In a large unbiased random DNA sequence, how...
Help with these two would be
appreciated
JUVIJVVEL In a large unbiased random DNA sequence, how often would you expect a 6 base targeting site specific restriction enzyme to cut? O On average every 4096 base pairs (446) O Never O On average every 1.296 base pairs (6^4) O Every 6 base pairs QUESTION 5 2 points Save Answer How do bacteria possessing restriction systems avoid targeting and cutting their own DNA? By methylating their chromosomal DNA O By methylating...
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Suppose the restriction enzyme Gsull binds a site that is 3 nucleotides in length. How often (how many base pairs) does this enzyme cut DNA? Your answer must be numeric.
A linear DNA molecule that is 3250 bp long is digested with restriction enzyme A alone, restriction enzyme B alone, or a combination of enzymes A and B to produce the following fragment sizes. Construct a restriction map of the DNA fragment. How many times does enzyme A cut the linear DNA molecule? If you are unable to place both enzymes A and B on the map, show one possible arrangement of enzyme A cognition sites on the DNA molecule.
Help with these two would be
appreciated
JUVIJVVEL In a large unbiased random DNA sequence, how often would you expect a 6 base targeting site specific restriction enzyme to cut? O On average every 4096 base pairs (446) O Never O On average every 1.296 base pairs (6^4) O Every 6 base pairs QUESTION 5 2 points Save Answer How do bacteria possessing restriction systems avoid targeting and cutting their own DNA? By methylating their chromosomal DNA O By methylating...
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