Homework Answers
ANSWER :
The C T G C A G nucleotides sequence will be recognised by a
G A C G T C
restriction enzyme because it cleaves them to produce two complementary strands of inverted repeat palindromic sequences (C T G C A G and G A C G T C).
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19) Which of the sequences below will be recognized by a restriction enzyme? TTTAG СССС AAATC...
The partial sequences recognized by two restriction enzymes “A” and “B” are indicated below. Complete the...
The partial sequences recognized by two restriction enzymes “A” and “B” are indicated below. Complete the sequences such that palindromes are generated for each of the sites. On your palindromes indicate which phosphodiester linkage would have to be cleaved such that 5’ protruding ends of enzyme “A” are compatible with protruding ends of enzyme “B”. Ex. CT/GCAG Enzyme “A”: 5’ATAG― ― ― ― 3’ Enzyme “B”: 5’GCG ― ― ― 3’ Consider your answer to the previous question. A DNA...
1.When cloning a PCR product into a plasmid using restriction enzymes, the restriction enzyme recognition sequences...
1.When cloning a PCR product into a plasmid using restriction enzymes, the restriction enzyme recognition sequences in the PCR product most likely came from _______, and the restriction enzyme recognition sequences in the plasmid most likely came from ________. a. A multiple cloning site / the primers b. The primers / a multiple cloning site c. Both came from primers d. Both came from the multiple cloning site e. Naturally present in the gene of interest / the multiple cloning...
A restriction map lists the locations of DNA sequences that are cut by a particular restriction...
A restriction map lists the
locations of DNA sequences that are cut by a particular restriction
enzyme for a piece of DNA, such as a chromosome or a plasmid.
Restriction maps are important when generating a construct for
experimental use. Digesting the DNA sequence with the restriction
enzymes will result in fragmented DNA of predictable sizes, based
on the restriction map, that allow a researcher to analyze if his
or her construct was generated correctly when visualized using gel
electrophoresis....
Show the resulting sequences when the sequence is cleaved by the restriction enzyme. Show the complementary...
Show the resulting sequences when the sequence is cleaved by the restriction enzyme. Show the complementary strand as well. i) BamHI 5’ ATCGGGATCCTA 3’ ii) BgIII 5’ GCTCTGCAGATCTA 3’?
You cut a gene of interest with HincII and would like to insert it into the...
You cut a gene of interest with HincII and would like to insert it into the multiple cloning site (MCS) of a plasmid. The plasmid's MCS contains the cut sites listed below. Which of these sites could you use to insert the gene of interest? Select all that apply. GTC*GAC 1. CAG*CTG G*TYRAC 2. CARYT*G GT*CGAC 3. CAGC*TG GGT*ACC 4. CCA*TGG GTA*TAC 5. CAT*ATG none of these sites
1. A circular plasmid has two PmeI restriction sites. A PmeI restriction enzyme will cut this...
1. A circular plasmid has two PmeI restriction sites. A PmeI restriction enzyme will cut this plasmid into two fragments. A. True B. False 2.In general, restriction enzymes that recognize four nucleotides have higher probability to produce more DNA fragments than those enzymes that recognize six nucleotides. A. true B. false 3. Which of the following sequences are palindromes? A. 5' TGGCCA 3' B. 5' GAAAAG 3' C. 5' CGATGG 3' D. 5' GACGAC 3' 4. Below are the possible...
Of the following steps to make recombinant DNA, which step occurs immediately after a restriction enzyme...
Of the following steps to make recombinant DNA, which step occurs immediately after a restriction enzyme recognizes specific nucleotide sequences?
14. A restriction enzyme (for example BamH1): (2 points) a. Is a specific sequence of DNA...
14. A restriction enzyme (for example BamH1): (2 points) a. Is a specific sequence of DNA b. Can be used to ligate DNA ends C. Recognizes palindromic DNA sequences d. Cuts random sequences of DNA
Question 19 (1 point) Saved Forward Primer: GTG ATG CGG GTT TGT GCA GAC (5' to...
Question 19 (1 point) Saved Forward Primer: GTG ATG CGG GTT TGT GCA GAC (5' to 3' on coding strand) Make a reverse primer from the following sequence, also from the coding strand, that is GC clamped and the Tm is within plus or minus 1 degree C (using the rule of thumb for calculating Tm we used in class). Please write the primer in its own 5' to 3' sequence, so we can send it out to be synthesized....
2.) Which of these is not a restriction enzyme? A) Bam HI B) HIndIII C) pAMP
2.) Which of these is not a restriction enzyme? A) Bam HI B) HIndIII C) pAMP
A restriction map lists the
locations of DNA sequences that are cut by a particular restriction
enzyme for a piece of DNA, such as a chromosome or a plasmid.
Restriction maps are important when generating a construct for
experimental use. Digesting the DNA sequence with the restriction
enzymes will result in fragmented DNA of predictable sizes, based
on the restriction map, that allow a researcher to analyze if his
or her construct was generated correctly when visualized using gel
electrophoresis....
14. A restriction enzyme (for example BamH1): (2 points) a. Is a specific sequence of DNA b. Can be used to ligate DNA ends C. Recognizes palindromic DNA sequences d. Cuts random sequences of DNA
Question 19 (1 point) Saved Forward Primer: GTG ATG CGG GTT TGT GCA GAC (5' to 3' on coding strand) Make a reverse primer from the following sequence, also from the coding strand, that is GC clamped and the Tm is within plus or minus 1 degree C (using the rule of thumb for calculating Tm we used in class). Please write the primer in its own 5' to 3' sequence, so we can send it out to be synthesized....
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