If it does code for a gene, what is the protein sequence encoded by this DNA...
- If it does code for a gene, what is the protein sequence encoded by this DNA sequence? Explain how you can tell.
- Are there any regulatory regions encoded by this DNA sequence? How can you tell?TTATGTATGTAGATGGGGCAGCTAACAGGGAGACTAAATTAGGAAAAGCAGGTTATGTTACTGACAGAGGAAGACAAAAGGTTGTTTCCATAACTGACACAACAAATCAGAAGACAGAGTTACAAGCAATTCATCTAGCTTTGCAGGATTCGGGATCAGAAGTAAACATAGTAACAGACTCACAATATGCATTAGGGATCATTCAAGCACAACCAGATAAAAGTGAATCAGAGTTAGTTAGCCAAATAATAGAGCAGTTAATAAATAAGGAAAAGATCTACCTGGCATGGGTACCAGCACATAAAGGAATTGGAGGAAATGAACAAGTAGATAAATTAGTTAGTGCTGGAGTCAGGAAAGTATAGTTT
Homework Answers
As TAG is the stop codon , protein translation stops here.
2. As there is no palindromic sequence, there is no regulatory sequence in the given DNA sequence.
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If it does code for a gene, what is the protein sequence
encoded by this DNA...
Here is a DNA sequence obtained from sequencing. Does this sequence code for a gene? How can...
Here is a DNA sequence obtained from sequencing. Does this sequence code for a gene? How can you tell? Is this a eukaryotic or prokaryotic gene? How can you tell? If it does code for a gene, what is the protein sequence encoded by this DNA sequence? Explain how you can tell. Are there any regulatory regions encoded by this DNA sequence? How can you tell?TTATGTATGTAGATGGGGCAGCTAACAGGGAGACTAAATTAGGAAAAGCAGGTTATGTTACTGACAGAGGAAGACAAAAGGTTGTTTCCATAACTGACACAACAAATCAGAAGACAGAGTTACAAGCAATTCATCTAGCTTTGCAGGATTCGGGATCAGAAGTAAACATAGTAACAGACTCACAATATGCATTAGGGATCATTCAAGCACAACCAGATAAAAGTGAATCAGAGTTAGTTAGCCAAATAATAGAGCAGTTAATAAATAAGGAAAAGATCTACCTGGCATGGGTACCAGCACATAAAGGAATTGGAGGAAATGAACAAGTAGATAAATTAGTTAGTGCTGGAGTCAGGAAAGTATAGTTT
1. Below is a DNA sequence that encodes a small, hypothetical protein. Assume there are no...
1. Below is a DNA sequence that encodes a small, hypothetical protein. Assume there are no introns in the coding sequence. Based on what you know about transcription, translation, and cellular trafficking, where would you expect the protein encoded by this gene to localize? Explain your reasoning. ATGATGTCATTCGTATCCCTGCTACTAGTGGGCATTCTGTTCTGGGCGACCGAGGCGGAA CAGCTGACCAAATGCGAAGTGTTTCAGATGGTCTCAAAGGGTGAAGAAGCTGAAGGGCGG CACTCGACAGGTGGCATGGATGAATTGTATAAGAAGGACGAGCTCTAA 2. If you were to mutate the abovementioned gene to disrupt localization of the encoded protein, what mutation(s) would you introduce? Where would you expect the protein to now localize?
A1. The following is the DNA sequence of a hypothetical gene for the SMALL protein. It...
A1. The following is the DNA sequence of a hypothetical gene for the SMALL protein. It is called the SMALL gene. i atgggattac actgtcacga ccaaatagcc ttcattgtat 41 caaaaggato aatcgagtta tag Imagine you are doing a research project in a laboratory and your supervisor asks you to clone the SMALL gene into the PBR322 plasmid (shown below). You must use the Pstl and EcoRI sites for your cloning. HindIII EcoRI | EcoRV BamHI 4359 0 29 185 4000 375 Sall Psti...
A DNA coding sequence of ATGCGTGGA(sequence for the rest of the gene)AATTAA encodes a protein chain...
A DNA coding sequence of ATGCGTGGA(sequence for the rest of the gene)AATTAA encodes a protein chain of 200 amino acids of MET-ARG-GLY-(196 more amino acids)-ASN after splicing. A mutation occurs in which the third G is changed to a T. Using the genetic code below, determine which type of mutation this is and briefly explain how translation will be affected for this protein chain.
What is the BRCA 1 gene, what is the known function of the encoded protein and...
What is the BRCA 1 gene, what is the known function of the encoded protein and how can you show it performs this function?
Match the protein listed below with the gene that encodes it or with the DNA sequence...
Match the protein listed below with the gene that encodes it or with the DNA sequence within the lac operon where it binds: lac repressor (encoded by...) [Choose] lac repressor (where it binds...) [Choose ] B-galactosidase [Choose] Lactose permease [Choose] Galactoside transacetylase [Choose] CAP [Choose] lacz lacy lacl Operator CAP site lacA
If you know the DNA sequence a gene, it is possible to determine the mRNA sequence...
If you know the DNA sequence a gene, it is possible to determine the mRNA sequence and the amino acid (Protein) sequence. If you only know the protein sequence, would you be able to accurately determine the mRNA sequence and DNA sequence? Explain.
Q1) You are studying a gene called ‘Muggle’ and the partial sequence of the encoded RNA...
Q1) You are studying a gene called ‘Muggle’ and the partial
sequence of the encoded RNA is given below:
5’ – CGACUAGAUGCAACGUCUUACGAACUAGG-3’
Write down the sequence for the antisense strand of DNA from
which this RNA has been transcribed. Make sure to clearly indicate
your 5’ and 3’ ends of the sequence.
Can this RNA fragment be used as a probe to detect the location
of the ‘Muggle’ gene on the chromosome? Briefly explain your
answer
• Two organisms have...
A reporter gene is an experimentally engineered regulatory DNA sequence from a gene of interest that...
A reporter gene is an experimentally engineered regulatory DNA sequence from a gene of interest that has been fused to a gene that encodes a protein that is easily observed experimentally. Why is this approach useful? a. It provides information on the binding interactions of the gene product. b. It provides information into where and when a gene is expressed. c. It can provide information as to where a gene is expressed. d. It can provide information as to when...
What is the role of the protein encoded by the lacZ gene? What is the role...
What is the role of the protein
encoded by the lacZ gene?
What is the role of the protein encoded by the lacZ gene? The lacZ gene encodes an enzyme that converts lactose to allolactose, and the lacZ gene O O O O encodes an enzyme that converts lactose to glucose and galactose. The lacZ gene encodes an enzyme that permits lactose to enter the bacterial cell. The lacZ gene encodes an enzyme that converts lactose to glucose and galactose....
A1. The following is the DNA sequence of a hypothetical gene for the SMALL protein. It is called the SMALL gene. i atgggattac actgtcacga ccaaatagcc ttcattgtat 41 caaaaggato aatcgagtta tag Imagine you are doing a research project in a laboratory and your supervisor asks you to clone the SMALL gene into the PBR322 plasmid (shown below). You must use the Pstl and EcoRI sites for your cloning. HindIII EcoRI | EcoRV BamHI 4359 0 29 185 4000 375 Sall Psti...
Match the protein listed below with the gene that encodes it or with the DNA sequence within the lac operon where it binds: lac repressor (encoded by...) [Choose] lac repressor (where it binds...) [Choose ] B-galactosidase [Choose] Lactose permease [Choose] Galactoside transacetylase [Choose] CAP [Choose] lacz lacy lacl Operator CAP site lacA
Q1) You are studying a gene called ‘Muggle’ and the partial
sequence of the encoded RNA is given below:
5’ – CGACUAGAUGCAACGUCUUACGAACUAGG-3’
Write down the sequence for the antisense strand of DNA from
which this RNA has been transcribed. Make sure to clearly indicate
your 5’ and 3’ ends of the sequence.
Can this RNA fragment be used as a probe to detect the location
of the ‘Muggle’ gene on the chromosome? Briefly explain your
answer
• Two organisms have...
What is the role of the protein
encoded by the lacZ gene?
What is the role of the protein encoded by the lacZ gene? The lacZ gene encodes an enzyme that converts lactose to allolactose, and the lacZ gene O O O O encodes an enzyme that converts lactose to glucose and galactose. The lacZ gene encodes an enzyme that permits lactose to enter the bacterial cell. The lacZ gene encodes an enzyme that converts lactose to glucose and galactose....
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